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| Status |
Public on Mar 01, 2023 |
| Title |
N-Myc overexpression leads to multiple types of acute leukemia in mice [RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The expression of the oncogene MYCN is upregulated in acute leukemia. However, the role of MYCN in leukemogenesis is poorly understood. We established multiple types of leukemia by retroviral overexpression of N-Myc in mice and studied the mechanism of leukemogenesis. Two types of hematopoietic stem cells and three types of hematopoietic progenitor cells highly purified from the bone marrow of adult C57BL/6 mice were transduced with N-Myc and transplanted into lethally irradiated mice. Acute leukemia developed from all these populations. Notably, not only B cell acute lymphoid leukemia, but also T cell acute lymphoid leukemia, acute myeloid leukemia, acute undifferentiated leukemia, and mixed phenotype acute leukemia developed, suggesting the multiple cells of origin, regardless types of leukemia. Moreover, leukemic stem cells were identified in Kit+Sca-1+Lin- bone marrow cells. RNA-seq data revealed the leukemia type-specific transcription profiles. Interestingly, acute undifferentiated leukemia appeared to be transcriptionally close to T cell acute lymphoid leukemia. Whole genome sequencing suggested that different types of leukemia arose associated with a large number of gene mutations induced by N-Myc overexpression but independent of retroviral integration sites. This study provides a new mouse model in which varying types of leukemia develop by only one oncogene.
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| Overall design |
Examination of mRNA profile for B-ALL, T-ALL, AML and AUL
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| Contributor(s) |
Bai H, Dong F, Ema H |
| Citation missing |
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| |
| Submission date |
Mar 04, 2020 |
| Last update date |
Mar 01, 2023 |
| Contact name |
Haitao Bai |
| E-mail(s) |
zhangqingyun@ihcams.ac.cn
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| Organization name |
Chinese Academy of Medical Sciences
|
| Department |
Institute of Hematology & Blood Diseases Hospital
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| Street address |
288 Nanjing Road
|
| City |
Tianjin |
| State/province |
Tianjin |
| ZIP/Postal code |
300020 |
| Country |
China |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE146333 |
N-Myc overexpression leads to multiple types of acute leukemia in mice |
|
| Relations |
| BioProject |
PRJNA610171 |
| SRA |
SRP251492 |
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