|
| Status |
Public on Oct 31, 2020 |
| Title |
RNA sequencing of Hes5.3+ cells from E5 chick embryonic retinas in control and retinoic acid treated conditions. |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Two E5 (HH26) chick embryonic retinas were dissected from two embryos, electroporated with Hes5.3-GFP reporter plasmid, and incubated in 2.5 μM retinoic acid (RA) in culture medium (DMEM, 10% FBS, 1% Penicillin/Streptomycin) for 8 hours. The corresponding opposite retinas from the same embryos were used as controls and incubated in culture medium with DMSO. Cells were dissociated and GFP+ cells were sorted by FACS. RNA was extracted using TRIzol reagent, according to manufacturer's protocol. RNA samples were quantified by Qubit 4 fluorometer (Thermo Fisher), and RNA quality was assessed by BioAnalyzer (Agilent). RNA sequencing was performed. This protocol was done in triplicate for RA and control conditions.
|
| |
|
| Overall design |
Gene expresson by RNA-seq in Hes5.3+ cells from two E5 chick embryonic retinas taken from two different embryos and treated with RA, vs two control retinas from the opposite side of the same embryos, in triplicate.
|
| |
|
| Contributor(s) |
Brodier L, Matter J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 05, 2020 |
| Last update date |
Oct 31, 2020 |
| Contact name |
Laurent Brodier |
| E-mail(s) |
laurent.brodier@unige.ch
|
| Organization name |
University of Geneva
|
| Department |
Molecular Biology
|
| Street address |
30 Quai Ernest-Ansermet
|
| City |
Geneva |
| State/province |
Geneva |
| ZIP/Postal code |
1211 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL23499 |
Illumina HiSeq 4000 (Gallus gallus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA610527 |
| SRA |
SRP251687 |
Less...
More...