We compared PBMC genomic response to exercise in both early (EG) and late-pubertal girls (LG)
Keywords: stress response
Overall design
Twenty healthy females (age range 8-17 y/o) participated in this study. A blood sample was taken before the onset of exercise and immediately after 30-min exercise bout. PBMCs were isolated using OptiPrepĀ® Density Gradient Medium (SIGMA). Total RNA was extracted using TRIzolĀ®. cRNA was hybridized onto Affymetrix U133+2 arrays (total of 40 chips).