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Series GSE146883 Query DataSets for GSE146883
Status Public on May 06, 2020
Title Peripheral Nerve Single-Cell Analysis Identifies Mesenchymal Ligands that Promote Axonal Growth (injured sciatic nerve microarray data)
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Peripheral nerves provide a supportive growth environment for developing and regenerating axons and are essential for maintenance and repair of many non-neural tissues. This capacity has largely been ascribed to paracrine factors secreted by nerve-resident Schwann cells. Here, we used single-cell transcriptional profiling to identify ligands made by different injured rodent nerve cell types and have combined this with cell-surface mass spectrometry to computationally model potential paracrine interactions with peripheral neurons. These analyses show that peripheral nerves make many ligands predicted to act on peripheral and CNS neurons, including known and previously uncharacterized ligands. While Schwann cells are an important ligand source within injured nerves, more than half of the predicted ligands are made by nerve-resident mesenchymal cells, including the endoneurial cells most closely associated with peripheral axons. At least three of these mesenchymal ligands, ANGPT1, CCL11, and VEGFC, promote growth when locally applied on sympathetic axons. These data therefore identify an unexpected paracrine role for nerve mesenchymal cells and suggest that multiple cell types contribute to creating a highly pro-growth environment for peripheral axons.
 
Overall design Distal sciatic nerve fragments from resected rat sciatic nerve were isolated from 4 biological replicates per experimental condition to determine the expression of ligand genes. Experimental conditions include 3 day post injured (dpi) nerve, 7 dpi nerve, and contralateral uninjured control sciatic nerve from animals of both the 3 dpi and 7 dpi conditions. Injuries were performed on 6 week old rats.
 
Contributor(s) Toma JS, Karamboulas K, Carr MJ, Kolaj A, Yuzwa SA, Mahmud N, Storer MA, Kaplan DR, Miller FD
Citation(s) 32349983
Submission date Mar 12, 2020
Last update date May 15, 2020
Contact name Freda Miller
E-mail(s) fredam@sickkids.ca
Organization name Hospital for Sick Children
Street address 686 Bay St
City Toronto
State/province ON
ZIP/Postal code M5G 0A4
Country Canada
 
Platforms (1)
GPL17117 [RaGene-2_0-st] Affymetrix Rat Gene 2.0 ST Array [transcript (gene) version]
Samples (16)
GSM4408722 Contralateral control uninjured sciatic nerve (3 days post injury [dpi]), biological replicate 1
GSM4408723 Contralateral control uninjured sciatic nerve (3 dpi), biological replicate 2
GSM4408724 Contralateral control uninjured sciatic nerve (3 dpi), biological replicate 3
This SubSeries is part of SuperSeries:
GSE146958 Peripheral Nerve Single-Cell Analysis Identifies Mesenchymal Ligands that Promote Axonal Growth
Relations
BioProject PRJNA612315

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE146883_RAW.tar 145.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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