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| Status |
Public on Apr 30, 2020 |
| Title |
Analyses of methylomes of Bones in cattle |
| Organism |
Bos taurus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
In this study, we generated whole genome bisulfite sequencing data of 2 samples for Bones in Holstein cattle. We analyzed the variations of DNA methylation among tissues compared to other tissues we generated before.
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| Overall design |
Genomic DNA for each tissue was isolated according to the QIAamp DNA Mini Kit protocol (QIAGEN, Valencia, CA, USA). The quality of DNA samples was evaluated using the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) including degradation, and potential RNA contamination, and purity (OD260/OD280), and concentration using a spectrophotometer (NanoDrop Technologies, Rockland, DE) to meet the requirements for library construction. The qualified genomic DNA from somatic tissues and sperm were used to construct a library. The library different lanes of a HiSeq X Ten (Illumina, San Diego, CA, USA) to generate 150-bp paired-end reads by Novogene (Novogene, Beijing, China).
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| Contributor(s) |
Liu S, Zhang S |
| Citation(s) |
32620158 |
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| Submission date |
Mar 18, 2020 |
| Last update date |
Aug 02, 2020 |
| Contact name |
Shengli Zhang |
| E-mail(s) |
zhangslcau@cau.edu.cn
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| Organization name |
China Agricultural University
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| Department |
Colledge of Animal Science and Techonology
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| Street address |
2rd, Yuanmingyuan West Road
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| City |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
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| Platforms (1) |
| GPL19172 |
Illumina HiSeq 2500 (Bos taurus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA613277 |
| SRA |
SRP253271 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE147184_RAW.tar |
563.9 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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