|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Mar 30, 2020 |
Title |
Analysis of transcriptomic regulation mechanisms underlying cucumber (Cucumis sativus L.) embryogenesis during ovary culture |
Organism |
Cucumis sativus |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
Background. Ovary culture has been a useful way to generate double haploid (DH) plant in cucumber (Cucumis sativus L.). However, the rate of embryo induction and the ability for induced embryo to grow into normal embryo are quite low. Moreover, the s mechanism of cucumber embryogenesis remains ambiguous. In this study, the molecular basis for cucumber embryogenesis was explored to set up basis for a more efficient ovary culture method. Differentially expressed genes during embryogenesis process, including the early stages of embryo formation, embryo maturation and shoot formation, were investigated using transcriptomic sequencing. Methods. Based on the cytological observation of cucumber ovary culture, the ovary culture can be divided into three stages:early embryo development, embryo maturation (from pre-embryos to cotyledon embryos) and the shoot formation stage. six key time points were selected for transcriptome sequencing and analysis. Results. We firstly conducted cytological observations which suggest that cell enlargement is the symbol for gametophytes to switch to sporophyte development pathway during early embryogenesis stage. In this stage, RNA-seq revealed 3468 up-regulated genes, including hormone signal transduction genes, hormone response genes and stress-induced genes. The reported embryogenesis-related genes BBM, HSP90 and AGL were also actively expressed during this stage. The total 480 genes that function in protein complex binding, microtubule binding, tetrapyrrole binding, tubulin binding and other microtubule activities were continuously up-regulated during the embryo maturation stage, indicating that the cytoskeleton structure was continuously being built and maintained by the action of microtubule-binding proteins and enzyme modification during embryo development. In shoot formation stage, 1383 genes were up-regulated, which were mainly enriched in phenylpropanoid biosynthesis, plant hormone signal transduction, phenylalanine metabolism, and starch and sucrose metabolism. The shoot formation stage might be regulated by 6 transcription factors that contained a B3 domain, 9 genes in the AP2/ERF family and 2 genes encoded WUS homologous domain proteins. Conclusions. These findings offer a valuable framework for explaining the transcriptional regulatory mechanism underlying embryogenesis during cucumber ovary culture.
|
|
|
Overall design |
samples were collected from six time-points of ovary culture and used three biological replicates for RNA-Seq analysis
|
|
|
Contributor(s) |
Deng Y, Fu W |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
|
Submission date |
Mar 20, 2020 |
Last update date |
Mar 31, 2020 |
Contact name |
LU YUXI |
E-mail(s) |
lyxrun@sina.com
|
Organization name |
Institute of Horticulture
|
Street address |
Guizhou Academy of Agricultural Sciences
|
City |
Guiyang |
ZIP/Postal code |
55006 |
Country |
China |
|
|
Platforms (1) |
GPL16310 |
Illumina HiSeq 2000 (Cucumis sativus) |
|
Samples (18)
|
|
Relations |
BioProject |
PRJNA613672 |
SRA |
SRP253481 |
Supplementary file |
Size |
Download |
File type/resource |
GSE147282_T0.txt.gz |
415.9 Kb |
(ftp)(http) |
TXT |
GSE147282_T1.txt.gz |
416.4 Kb |
(ftp)(http) |
TXT |
GSE147282_T2.txt.gz |
407.1 Kb |
(ftp)(http) |
TXT |
GSE147282_T3.txt.gz |
417.6 Kb |
(ftp)(http) |
TXT |
GSE147282_T4.txt.gz |
422.1 Kb |
(ftp)(http) |
TXT |
GSE147282_T5.txt.gz |
421.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
|
|
|
|
|