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| Status |
Public on Feb 09, 2023 |
| Title |
An interaction between Gβγ and RNA polymerase II regulates transcription in cardiac fibroblasts |
| Organism |
Rattus norvegicus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Gβγ subunits are involved in an array of different signalling processes in various compartments of the cell, including the nucleus. To gain further insight into the functions of nuclear Gβγ, we investigated the functional role of Gβγ signalling in the regulation of GPCR-mediated gene expression in primary rat neonatal cardiac fibroblasts. Here, we demonstrate that following activation of the angiotensin II type I receptor in these cells, Gβγ dimers interact with RNA polymerase II (RNAPII). Our findings suggest that Gβ1 recruitment to RNAPII negatively regulates the fibrotic transcriptional response, which can be overcome by strong fibrotic stimuli. The Gβγ-RNAPII interaction was also investigated in HEK 293 cells and was regulated by signaling pathways that diverged from those operating in cardiac fibroblasts, suggesting that although it may be a conserved feature of transcriptional regulation, such regulation may be cell-specific. Taken together, our studies reveal a novel interaction between Gβγ subunits and RNA polymerase II and expand the role for Gβγ signalling in cardiac fibrosis.
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| |
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| Overall design |
Gβ1 and RNAPII ChIP-seq from neonatal rat cardiac fibroblasts
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| Contributor(s) |
Khan SM, Martin RD, Gora S, Bouazza C, Jones-Tabah J, Zhang A, MacKinnon S, Trieu P, Clarke PS, Tanny JC, Hébert TE |
| Citation(s) |
36841480 |
| |
| Submission date |
Mar 23, 2020 |
| Last update date |
Apr 04, 2023 |
| Contact name |
Terence Hébert |
| E-mail(s) |
terence.hebert@mcgill.ca
|
| Organization name |
McGill University
|
| Department |
Pharmacology and Therapeutics
|
| Street address |
3655 Promenade Sir William Osler
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3G1Y6 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL22396 |
Illumina HiSeq 4000 (Rattus norvegicus) |
|
| Samples (17)
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| Relations |
| BioProject |
PRJNA614512 |
| SRA |
SRP253756 |
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