|
| Status |
Public on Dec 08, 2020 |
| Title |
In situ HiC of primary mouse B cell progenitor populations after activation with LPS |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Naive resting B-cells were purified from the bone marrow of C57BL/6 mice using flow cytometry sorting. Naive B cells were activated with LPS. Activated but undivided cells were harvested 3 hours, 10 hours or 33 hours after the LPS treatment. The Fluorescent Ubiquitination-based Cell Cycle Indicator (FUCCI) system was also used to isolate imminently dividing B cells specifically in the G1 stage of the cell cycle prior to the S phase.
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| |
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| Overall design |
Two biological replicates were prepared at each activation time. In situ Hi-C libraries were sequenced on an Illumina NextSeq 500 to produce paired-end 81bp reads.
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| |
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| Contributor(s) |
Coughlan HD, Smyth GK |
| Citation(s) |
33637722 |
| |
| Submission date |
Mar 24, 2020 |
| Last update date |
Mar 09, 2021 |
| Contact name |
Gordon K Smyth |
| E-mail(s) |
smyth@wehi.edu.au
|
| Phone |
(+61 3) 9345 2326
|
| Fax |
(+61 3) 9347 0852
|
| URL |
http://www.wehi.edu.au
|
| Organization name |
Walter and Eliza Hall Institute of Medical Research
|
| Department |
Bioinformatics
|
| Lab |
Smyth
|
| Street address |
1G Royal Pde
|
| City |
Parkville |
| State/province |
Vic |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE147497 |
RNA-seq and in situ Hi-C of primary mouse B cell progenitor populations after activation with LPS |
|
| Relations |
| BioProject |
PRJNA614943 |
| SRA |
SRP253877 |
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