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Series GSE147467 Query DataSets for GSE147467
Status Public on Dec 08, 2020
Title In situ HiC of primary mouse B cell progenitor populations after activation with LPS
Organism Mus musculus
Experiment type Other
Summary Naive resting B-cells were purified from the bone marrow of C57BL/6 mice using flow cytometry sorting. Naive B cells were activated with LPS. Activated but undivided cells were harvested 3 hours, 10 hours or 33 hours after the LPS treatment. The Fluorescent Ubiquitination-based Cell Cycle Indicator (FUCCI) system was also used to isolate imminently dividing B cells specifically in the G1 stage of the cell cycle prior to the S phase.
Overall design Two biological replicates were prepared at each activation time. In situ Hi-C libraries were sequenced on an Illumina NextSeq 500 to produce paired-end 81bp reads.
Contributor(s) Coughlan HD, Smyth GK
Citation(s) 33637722
Submission date Mar 24, 2020
Last update date Mar 09, 2021
Contact name Gordon K Smyth
Phone (+61 3) 9345 2326
Fax (+61 3) 9347 0852
Organization name Walter and Eliza Hall Institute of Medical Research
Department Bioinformatics
Lab Smyth
Street address 1G Royal Pde
City Parkville
State/province Vic
ZIP/Postal code 3052
Country Australia
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM4431512 B cell activation 3 hours 1
GSM4431513 B cell activation 3 hours 2
GSM4431514 B cell activation 10 hours 1
This SubSeries is part of SuperSeries:
GSE147497 RNA-seq and in situ Hi-C of primary mouse B cell progenitor populations after activation with LPS
BioProject PRJNA614943
SRA SRP253877

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Supplementary file Size Download File type/resource
GSE147467_RAW.tar 1.4 Gb (http)(custom) TAR (of MTX)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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