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| Status |
Public on May 20, 2020 |
| Title |
Hedgehog-regulated genes in the chick coelomic epithelium |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In the experimental group, the coelomic cavity of HHStage 12-13 chick embryos was electroporated with a plasmid expressing PatchedDLoop2 (a dominant negative hedgehog pathway inhibitor) and GFP. In the control group, a plasmid expressing GFP alone was electroporated. After 14 hours, embryos were dissociated into single cells, FACS sorted, and processed for RNAseq.
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| Overall design |
5 experimental and 4 controls were analyzed. These were all separate biological replicates.
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| |
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| Contributor(s) |
Arraf AA, Yelin R, Reshef I, Schneider J, Vladimirov FK, Schultheiss TM |
| Citation(s) |
32437643 |
| |
| Submission date |
Mar 25, 2020 |
| Last update date |
Aug 19, 2020 |
| Contact name |
Thomas Schultheiss |
| E-mail(s) |
TSCHULTH@TECHNION.AC.IL
|
| Organization name |
Technion-Israel Institute of Technology
|
| Department |
Faculty of Medicine
|
| Street address |
Efron 1
|
| City |
Haifa |
| ZIP/Postal code |
31096 |
| Country |
Israel |
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| Platforms (1) |
| GPL19005 |
Illumina HiSeq 2500 (Gallus gallus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA615212 |
| SRA |
SRP254040 |
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