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| Status |
Public on Aug 11, 2020 |
| Title |
The miRNA-mRNA Interactome of murine iPSC-derived Chondrocytes in Response to Inflammatory Cytokines |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
The goal of this study was to understand the mechanisms of miRNA regulation of the transcriptome of tissue engineered cartilage in response to IL-1β and TNF-α using an in vitro murine induced pluripotent stem cell (miPSC) model system. miPSCs were chondrogenically differentiated for 21 days and then treated with 1 ng/mL of IL-1β, 20 ng/mL of TNF-α, or control media without cytokines for 0, 4, 12, 24, and 72 hours. We performed an integrative analysis of miRNA- and RNA-sequencing data to determine the temporal and dynamic responses of miRNAs and genes to specific inflammatory cytokines. Through integration of mRNA and miRNA sequencing data, we created networks of miRNA-mRNA interactions which may be controlling the response to inflammatory cytokines.
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| Overall design |
miRNA and mRNA profiles of tissue-engineered cartilage; in triplicate, using a Illumina HiSeq3000
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| Contributor(s) |
Ross A, Guilak F |
| Citation(s) |
32767602 |
| |
| Submission date |
Mar 31, 2020 |
| Last update date |
Aug 11, 2020 |
| Contact name |
Alison Ross |
| Organization name |
Washington University in St. Louis
|
| Street address |
Couch Biomedical Research Building
|
| City |
Saint Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
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| Samples (78)
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| Relations |
| BioProject |
PRJNA622234 |
| SRA |
SRP254785 |
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