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Series GSE147870 Query DataSets for GSE147870
Status Public on Apr 21, 2021
Title Conservation and divergence of vulnerability and responses to stressors between human and mouse astrocytes
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Astrocytes were purified from mouse cortices and human cortex tissue by immunopanning with HepaCAM. All treatments were proceeded on 3DIV in serum-free medium. Astrocytes were treated by TNFα for 48hr, by Poly I:C for 72hr, or by hypoxia for 72hr. Acutely purified atrocytes RNA was harvest immediately after HepaCAM immunopanning. Serum selected astrocytes RNA was collected after 4-6 days culturing in serum medium. Serum-free cultured astrocytes RNA was collected on 5-6 DIV. Serum-selection purified human astrocytes were transplanted into P2-11 Rag2 immunodeficient mouse brains, and after about 8 months, transplanted human and host mouse astocytes RNA was collected acutely on HepaCAM immunopanning dish.
 
Overall design For each kind of treatments and relative controls, there are four patient samples. Six TNFα treated mouse samples and six relative control samples. Four Poly I:C treated mouse samples and four relative control samples. Four hypoxia treated mouse samples and four relative control samples. There are four acutely purified human samples from four patients, three serum selected human astrocyte samples from three patients, and five serum-free cultured samples from five patients.. Three transplanted human samples from three patients and three mouse host samples.
 
Contributor(s) Li J, Zhang Y
Citation(s) 34172753
Submission date Apr 01, 2020
Last update date Jun 28, 2021
Contact name Jiwen Li
E-mail(s) ljwen@ucla.edu
Organization name UCLA
Street address 635 Charles E. Young Dr. S.
City Los Angeles
ZIP/Postal code 90095
Country USA
 
Platforms (4)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (77)
GSM4447414 serum_selected_rep1 [serum selected-BC1]
GSM4447415 serum_selected_rep2 [serum selected-BC4]
GSM4447416 serum_selected_rep3 [serum selected-BC8]
Relations
BioProject PRJNA622439
SRA SRP254887

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Supplementary file Size Download File type/resource
GSE147870_RPKM.xlsx 42.2 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
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