|
| Status |
Public on Sep 01, 2023 |
| Title |
Microglia coordinate the anti-tumor immune response to breast cancer brain metastasis [Foxn1 nu/nu 10X data] |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used single-cell RNA-sequencing to investigate the role of brain-resident microglia versus infiltrating cells in a mouse model of breast cancer brain metastasis (BCBM). We generated brain metastases with a cardiac-injection of the human triple-negative breast cancer cell line MDA-MB-231BR2 (231BR) into Foxn1 nu/nu mice and allowed tumors to grow in the brain for four weeks. We then compared astrocytes (ASCA2+CD45-) and myeloid cells (CD11b+CD45+) from these mice verses control Foxn1 nu/nu brains to find transcriptional changes associated with BCBM.
|
| |
|
| Overall design |
Single-cell RNA-Seq of myeloid cells, astrocytes, and MDA-MB-231BR2 (231BR) from the brains of Foxn1 nu/nu mice with and without breast cancer brain metastases.
|
| |
|
| Contributor(s) |
Lawson DA, Blake K |
| Citation(s) |
37957324 |
| |
| Submission date |
Apr 01, 2020 |
| Last update date |
Dec 04, 2023 |
| Contact name |
Devon Lawson |
| Organization name |
University of California, Irvine
|
| Street address |
839 Health Science Road, Sprague Hall 140
|
| City |
Irvine |
| State/province |
CA |
| ZIP/Postal code |
92612 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
| GPL25431 |
Illumina HiSeq 4000 (Homo sapiens; Mus musculus) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE147949 |
Microglia coordinate the anti-tumor immune response to breast cancer brain metastasis |
|
| Relations |
| BioProject |
PRJNA622588 |
| SRA |
SRP254950 |
Less...
More...