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| Status |
Public on May 12, 2020 |
| Title |
poly(UG)-tailed RNAs in Genome Protection and Epigenetic Inheritance [ncRNA-Seq] |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Mobile genetic elements threaten genome integrity in all organisms. MUT-2/RDE-3 is a ribonucleotidyltransferase required for transposon silencing and RNA interference (RNAi) in C. elegans. When tethered to RNAs in heterologous expression systems, RDE-3 can add long stretches of alternating non-templated uridine (U) and guanosine (G) ribonucleotides to the 3’ termini of these RNAs (polyUG or pUG tails). Here, we show that, in its natural context in C. elegans, RDE-3 adds pUG tails to transposon RNAs, as well as to targets of RNAi. pUG tails with more than 16 perfectly alternating 3’ U and G nucleotides convert RNA fragments into agents of gene silencing. pUG tails promote gene silencing by recruiting RNA-dependent RNA Polymerases (RdRPs), which use pUG-tailed RNAs (pUG RNAs) as templates to synthesize small interfering RNAs (siRNAs). Our results show that cycles of pUG RNA-templated siRNA synthesis and siRNA-directed mRNA pUGylation underlie dsRNA-directed transgenerational epigenetic inheritance in the C. elegans germline. We speculate that this pUG RNA/siRNA silencing loop allows parents to inoculate progeny against the expression of unwanted or parasitic genetic elements.
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| Overall design |
Small RNA-seq on 2 biological replicates of rde-1(ne219); oma-1(zu405) animals injected with either an oma-1 pGC RNA or an oma-1 pUG RNA. Noninjected animals serve as negative controls.
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| Web link |
https://doi.org/10.1038/s41586-020-2323-8
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| |
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| Contributor(s) |
Yan J, Fei Y, Kennedy S |
| Citation(s) |
32499657 |
| |
| Submission date |
Apr 03, 2020 |
| Last update date |
Aug 31, 2021 |
| Contact name |
Aditi Shukla |
| E-mail(s) |
aditishukla@fas.harvard.edu
|
| Phone |
6174321235
|
| Organization name |
Harvard Medical School
|
| Department |
Genetics
|
| Lab |
Kennedy
|
| Street address |
77 Avenue Louis Pasteur, NRB 266
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19757 |
Illumina NextSeq 500 (Caenorhabditis elegans) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE148134 |
poly(UG)-tailed RNAs in Genome Protection and Epigenetic Inheritance |
|
| Relations |
| BioProject |
PRJNA623003 |
| SRA |
SRP255189 |
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