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Series GSE148198 Query DataSets for GSE148198
Status Public on Dec 11, 2020
Title MicroRNA-802 regulates the innate immune response through Paneth cell expansion and Wnt signaling
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Paneth cells are important for maintaining epithelial cell renewal and modulating innate immune function in the intestine through secretion of growth factors and antimicrobial peptides, which help sustain epithelial stem and progenitor cells and contribute to the intestinal barrier and protection against pathogenic bacteria. Here we show that the intestine-enriched miR-802 is a central regulator of intestinal epithelial cell proliferation and Paneth cell function. Genetic ablation of mir-802 in mice leads to amplified ROS generation and Notch/Wnt signaling, increased intestinal epithelial turnover, impaired enterocyte differentiation and nutrient uptake, and increased enterocyte apoptosis. Mice lacking mir-802 in the intestine also exhibit Paneth cell expansion, increased antimicrobial peptide production, and protection against Salmonella infection. This phenotype relies on the miR-802 target gene Tmed9.
Overall design In total, 6 samples from isolated jejunum enterocyte cells were analyzed. From these, 3 were controls (mir-802fl/fl) and the other 3 were intestine specific KO (Vil-Cre mir-802fl/fl).
Contributor(s) Stoffel M, Yagabasan B
Citation(s) 34099655
Submission date Apr 06, 2020
Last update date Jun 15, 2021
Contact name Algera Goga
Organization name ETH Zurich
Department Biology
Lab Markus Stoffel
Street address Otto-Sttern-Weg 7
City Zurich
State/province Schweiz
ZIP/Postal code Zurich 8093
Country Switzerland
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (6)
GSM4456003 RNAseq_28 Vil-Cre mir-802fl/fl
GSM4456004 RNAseq_31 mir-802fl/fl
GSM4456005 RNAseq_33 Vil-Cre mir-802fl/fl
BioProject PRJNA623451
SRA SRP255506

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Supplementary file Size Download File type/resource
GSE148198_RAW.tar 8.3 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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