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| Status |
Public on Apr 08, 2020 |
| Title |
Delineation and modulation of the natural killer cell transcriptome in rhesus macaques during ZIKV and SIV infections |
| Organism |
Macaca mulatta |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Natural killer (NK) cells are crucial regulators of antiviral and anti-tumor immune responses. Although in humans some NK cell transcriptional programs are relatively well established, NK cell transcriptional networks in non-human primates (NHP) remain poorly delineated. Here we performed RNA-Seq experiments using purified NK cells from experimentally naïve rhesus macaques, providing the first transcriptional characterization of pure NK cells in a NHP species. This novel NK cell transcriptomic signature (NK RMtsig) overlaps with published human NK signatures, allowing us to identify new key signaling and transcription factor networks underlying NK cell function. Finally, we show that applying NK RMtsig to an unrelated rhesus macaque cohort infected with SIVmac251 or ZIKV can sensitively detect NK cell repertoire perturbations, thus confirming applicability of this approach. In sum, we propose this NHP NK cell signature will serve as a useful resource for future studies involving infection, disease or treatment modalities in NHP
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| |
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| Overall design |
Examination of the baseline transcriptome of NK cells from 6 rhesus macaque.
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| |
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| Contributor(s) |
Aid M, Ram D, Bosinger S, Barouch DH, Reeves RK, Tharp GK |
| Citation(s) |
32411625 |
| |
| Submission date |
Apr 08, 2020 |
| Last update date |
May 18, 2020 |
| Contact name |
Gregory K Tharp |
| E-mail(s) |
gktharp@emory.edu
|
| Phone |
404-727-7797
|
| Organization name |
Yerkes National Primate Research Center
|
| Department |
Developmental and Cognitive Neuroscience
|
| Lab |
Genomics Core
|
| Street address |
954 Gatewood Dr
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30329-4208 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL23804 |
Illumina HiSeq 3000 (Macaca mulatta) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA623754 |
| SRA |
SRP255707 |
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