NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE148618 Query DataSets for GSE148618
Status Public on May 01, 2020
Title Impact of drought and plant litter chemistry on microbial gene expression
Organism leaf litter metagenome
Experiment type Expression profiling by high throughput sequencing
Summary Drought represents a significant stress to microorganisms and is known to reduce microbial activity and organic matter decomposition in Mediterranean ecosystems. However, we lack a detailed understanding of the drought stress response of microbial decomposers. Here we present metatranscriptomic data on the physiological response of in situ microbial communities on plant litter to long-term drought in Californian grass and shrub ecosystems.
 
Overall design Litter was collected in summer on 30 August 2017 from all four replicated plots within a treatment and homogenized by hand-mixing. Litter bags were made by placing 6 g dry litter mass into 15 cm x15 cm bags made from 1 mm mesh window screen. Bags were deployed on 12 September 2017. An in-situ pulse wetting was performed on 30 November 2017 to simulate a discrete rainstorm of ~13 mm using previously collected rainwater. Litter bags were collected before wetting (Sampling 1) and another batch of bags was collected the following day (Sampling 2). There was no precipitation in the following days and therefore the litter dried to pre-wetting moisture levels. We collected another batch 12 days after wetting (Sampling 3). Sixteen litter bags were sampled at each time point (2 vegetation types x 2 precipitation treatments x 4 replicates). Retrieved litter bags were immediately transported to the lab at room temperature.
 
Contributor(s) Malik AA
Citation(s) 32444813
Submission date Apr 14, 2020
Last update date Nov 09, 2020
Contact name Ashish Malik
E-mail(s) planetofashish@gmail.com
Organization name University of Aberdeen
Street address St Machar drive
City Aberdeen
ZIP/Postal code AB24 3UU
Country United Kingdom
 
Platforms (1)
GPL28389 Illumina HiSeq 4000 (leaf litter metagenome)
Samples (41)
GSM4475199 SADRT1
GSM4475200 SADRT2
GSM4475201 SADRT3
Relations
BioProject PRJNA625207
SRA SRP256318

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE148618_SA_DTR_GENE_ABUN.csv.gz 386.5 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.