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| Status |
Public on Aug 24, 2021 |
| Title |
CRISPR library knockout screening identified candidate genes whose loss of function confers lapatinib resistance in HER2-amplified GC cells. |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
To identify genes, whose loss of function confers drug resistance to lapatinib, we performed a genome-wide CRISPR/Cas9 gene knockout screening in two human gastric cancer cell lines harboring HER2 amplification, N87 and OE19, respectively. By performing this study, we identiied a goup of genes associated with lapatinib resistance in HER2-amplified gastric cancer.
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| Overall design |
A pooled GeCKO V2 library were amplified for lentivirus production, and then two biological replicates of N87 and OE19 cells were transduced with the lentivirus containing GeCKO V2 library at a multiplicity of infection (MOI) of 0.3. Puromycin selection was performed before lapatinib treatment on each cell line to enrich successfully transduced cells. After 14 days of lapatinib treatment, the drug-treated and vehicle-treated cells were harvested. Genomic DNA was extracted for PCR amplification and subsequent deep sequencing of the regions containing the sgRNAs.
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| Contributor(s) |
Ning G, Zhu Q |
| Citation(s) |
34399705 |
| Submission date |
Apr 14, 2020 |
| Last update date |
Aug 24, 2021 |
| Contact name |
Charles Lee |
| E-mail(s) |
Charles.lee@jax.org
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| Organization name |
The Jackson Laboratory
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| Street address |
10 Discovery Dr
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| City |
Farmington |
| State/province |
Connecticut |
| ZIP/Postal code |
06032 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA625316 |
| SRA |
SRP256373 |
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