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| Status |
Public on Jun 30, 2021 |
| Title |
Amnion signals are essential for mesoderm formation in primates |
| Organism |
Macaca fascicularis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Embryonic development is largely conserved among mammals. However, certain genes show divergent functions. By generating a transcriptional atlas containing >30,000 cells from post-implantation non-human primate embryos, we uncover that ISL1, a gene with a well-established role in cardiogenesis, controls a gene regulatory network in primate amnion. CRISPR/Cas9-targeting of ISL1 results in non-human primate embryos which do not yield viable offspring, demonstrating that ISL1 is critically required in primate embryogenesis. On a cellular level, mutant ISL1 embryos display a failure in mesoderm formation due to reduced BMP4 signaling from the amnion. Via loss of function and rescue studies in human embryonic stem cells we confirm a similar role of ISL1 in human in vitro derived amnion. This study highlights the importance of the amnion as a signaling center during primate mesoderm formation and demonstrates the potential of in vitro primate model systems to dissect the genetics of early human embryonic development.
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| Overall design |
Single cell gene expression profiling (mRNA) of in vitro cultured wildtype and ISL1 hypomorphic mutant cynomologus monkey embryos at Day 10, Day 12 and Day 14. WT-batch 1: D10 2 embryos, D12 1 embryo, D14 2 embyos; WT-batch 2: D10 3 embryos, D12 2 embryos, D14 2 embryos; ISL1-mutant batch-1: D10 3 embryos, D12 3 embryos, D14 3 embryos; ISL1-mutant batch-2: D12 1 embryo, D14 1 embryo
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| Contributor(s) |
Yang R, Goedel A, Chien KR |
| Citation(s) |
34446705 |
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| Submission date |
Apr 14, 2020 |
| Last update date |
Aug 30, 2021 |
| Contact name |
Alexander Goedel |
| E-mail(s) |
alexander.goedel@ki.se
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| Organization name |
Karolinska Institutet
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| Department |
Department of Cell and Molecular Biology (CMB)
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| Lab |
Kenneth Chien
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| Street address |
Solnavägen 9
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| City |
Solna |
| ZIP/Postal code |
171 65 |
| Country |
Sweden |
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| Platforms (1) |
| GPL28212 |
Illumina NovaSeq 6000 (Macaca fascicularis) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA625334 |
| SRA |
SRP256390 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE148683_CM_filtered_SoupX_corrected.tsv.gz |
115.3 Mb |
(ftp)(http) |
TSV |
| GSE148683_CM_filtered_uncorrected.tsv.gz |
117.0 Mb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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