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| Status |
Public on Apr 17, 2021 |
| Title |
The g-tubulin meshwork assists to the transport of cytosolic PCNA to chromatin in mammalian cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Changes in the location of g-tubulin ensure cell survival and preserve genome integrity. We investigated whether the nuclear accumulation of g-tubulin facilitates the transport of proliferating cell nuclear antigen (PCNA) between the cytosolic and the nuclear compartment in mammalian cells. We found that the g-tubulin meshwork assists to the recruitment of PCNA to chromatin. Also, decreased levels of g-tubulin reduce the nuclear pool of PCNA. In addition, the g-tubulin C terminus encodes a PCNA-interacting peptide (PIP) motif, and a g-tubulin–PIP-mutant affects the nuclear accumulation of PCNA. In a cell-free system, PCNA and g-tubulin formed a complex. In tumors, there is a significant positive correlation between TUBG1 and PCNA expression. Thus, we report a novel mechanism that constitutes the basis for tumor growth by which the g-tubulin meshwork maintains indefinite proliferation by acting as an opportune scaffold for the transport of PCNA from the cytosol to the chromatin.
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| Overall design |
The experiment was preformed in one cell line and a total number of 22 samples were included.There are two samples for each antibody (gamma tubulin, macrohistone2A and PCNA). For gamma tubulin rhere is 2 samples for G0/G1, 0h, 3h, 5h, 7h, 9h and for MH2A and PCNA the timepoints are 3h and 5h and as control, there are 2 whole cell lysate (Input) samples included.
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| Contributor(s) |
Kristensson MA |
| Citation(s) |
34158617 |
| |
| Submission date |
Apr 16, 2020 |
| Last update date |
Jul 18, 2021 |
| Contact name |
Maria Alvarado-Kristensson |
| Organization name |
Lund University
|
| Street address |
59 Jan Waldenstr ms street
|
| City |
Lund |
| ZIP/Postal code |
20502 Malm |
| Country |
Sweden |
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| Platforms (1) |
| GPL16558 |
AB 5500 Genetic Analyzer (Homo sapiens) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA625747 |
| SRA |
SRP256642 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE148798_MH2A_3h.bedgraph.gz |
4.7 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE148798_MH2A_5h.bedgraph.gz |
543.0 Kb |
(ftp)(http) |
BEDGRAPH |
| GSE148798_PCNA_3h.bedgraph.gz |
229.8 Kb |
(ftp)(http) |
BEDGRAPH |
| GSE148798_PCNA_5h.bedgraph.gz |
334.2 Kb |
(ftp)(http) |
BEDGRAPH |
| GSE148798_gTub_0h.bedgraph.gz |
116.2 Kb |
(ftp)(http) |
BEDGRAPH |
| GSE148798_gTub_3h.bedgraph.gz |
210.7 Kb |
(ftp)(http) |
BEDGRAPH |
| GSE148798_gTub_5h.bedgraph.gz |
351.5 Kb |
(ftp)(http) |
BEDGRAPH |
| GSE148798_gTub_7h.bedgraph.gz |
349.7 Kb |
(ftp)(http) |
BEDGRAPH |
| GSE148798_gTub_9h.bedgraph.gz |
18.4 Kb |
(ftp)(http) |
BEDGRAPH |
| GSE148798_gTub_G0_G1.bedgraph.gz |
55.7 Kb |
(ftp)(http) |
BEDGRAPH |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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