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Series GSE148966 Query DataSets for GSE148966
Status Public on Apr 18, 2021
Title Identification of M3 muscarinic acetylcholine receptor reactive Th17 cells in patients with primary Sjögren’s syndrome
Organism Homo sapiens
Experiment type Other
Summary Previously M3 muscarinic acetylcholine receptore (M3R) reactive Th1 cells were identified, and here, M3R reactive Th17 cells were identified in peripheral blood of primary Sjögren’s syndrome patients using ELISpot method. To assess TCR repertoire overlap between identified M3R reactive Th17 cells, and salivary gland infiltrating T cells, we performed high throughput TCR sequencing of those cells from pSS patient.
 
Overall design From pSS patient who were detected positive for M3R reactive Th17 cells by ELISpot method, IL-17 producing CD4+ T cells were FACS sorted after stimulating with M3R peptide for 8 days. Salivary gland(SG) was taken at the time of diagnosis, and stored in RNA later solution. Then, we prepared libraries from these CD4+ T cell sample, together with salivary gland sample and performed unbiased high throughput TCR sequencing using an Ion Genestudio S5 next generation sequencer.
 
Contributor(s) Aoki H, Shichino S, Matsushima K, Abe S, Takahashi H, Tsuboi H, Matsumoto I, Sumida T
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Submission date Apr 20, 2020
Last update date Apr 19, 2021
Contact name Shigeyuki Shichino
E-mail(s) s_shichino@rs.tus.ac.jp
Organization name Tokyo University of Science
Department Department of Molecular regulation of Inflammatory and Immune Diseases
Street address 2641, Yamasaki, Noda-shi
City Chiba
ZIP/Postal code 278-0022
Country Japan
 
Platforms (1)
GPL23934 Ion Torrent S5 (Homo sapiens)
Samples (3)
GSM4486771 SG_TCRb
GSM4486772 PBMC_CD4posIL17pos_TCRb
GSM4486773 PBMC_CD4posIL17neg_TCRb
Relations
BioProject PRJNA626633
SRA SRP257554

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE148966_RAW.tar 1.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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