|
| Status |
Public on Jun 10, 2021 |
| Title |
Genome-wide maps of EED and H3K27me3 binding genes in WT and HBL1 KO human induced pluripotent stem cells (hiPSCs). |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We reported loss of HBL1 lnRNA promoted cardiogenesis and the interaction of HBL1-EED in hiPSCs. Here we used specific EED and H3K27me3 antibodies to pull down their binding genomic DNAs in hiPSCs, respectively, which let us know the potential genes regulated by HBL1-EED-H3K27me3 during cardiogenesis. 1% Input samples were collected from the same samples after chromatin shearing.
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| |
|
| Overall design |
Examination of EED and H3K27me3 binding genes in hiPSCs.
|
| |
|
| Contributor(s) |
Yang L, Liu J |
| Citation(s) |
34027990 |
| |
| Submission date |
Apr 24, 2020 |
| Last update date |
Jul 27, 2021 |
| Contact name |
Lei Yang |
| E-mail(s) |
lyang7@iu.edu
|
| Phone |
(317) 278-5233
|
| Organization name |
Indiana University School of Medicine
|
| Department |
Department of Pediatrics
|
| Street address |
1044 W. Walnut St.
|
| City |
Indianapolis |
| State/province |
Indiana |
| ZIP/Postal code |
46202 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE149409 |
LncRNA HBL1 Recruits Polycomb Repressive Complex 2 on Essential Cardiogenic Genes to Orchestrate Human Cardiogenesis |
|
| Relations |
| BioProject |
PRJNA627955 |
| SRA |
SRP258363 |
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