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| Status |
Public on Apr 29, 2020 |
| Title |
Analysis of global gene expressions in initial infection of Mycobacterium avium subsp. paratuberculosis using epithelial cells-peripheral blood mononuclear cells culture passage model. |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: Understanding host-MAP interactions in initial stage of infection
Methods: Native MAP and MDBK processed (4h) MAP were infected to bovine PBMCs. Total RNA after 24h and 72h were harvested. RNA-Seq was performed using total RNA of each group.
Result: The gene expression patterns commonly activated in both groups suggest that Th17 activation is the main immunological response to MAP infection.
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| |
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| Overall design |
in vitro epithelial cells-peripheral blood mononuclear cells (PBMCs) culture passage model to establish a host-MAP interaction during initial stage of infection.
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| |
|
| Contributor(s) |
Park H, Yoo H |
| Citation(s) |
33273606 |
| Submission date |
Apr 28, 2020 |
| Last update date |
Dec 08, 2020 |
| Contact name |
Hong-Tae Park |
| E-mail(s) |
twinstar23@snu.ac.kr
|
| Organization name |
Seoul National University
|
| Lab |
Infectious diseases
|
| Street address |
1, Gwanak-ro
|
| City |
Seoul |
| ZIP/Postal code |
08826 |
| Country |
South Korea |
| |
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| Platforms (1) |
| GPL26012 |
Illumina NovaSeq 6000 (Bos taurus) |
|
| Samples (12)
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|
| Relations |
| BioProject |
PRJNA628877 |
| SRA |
SRP258878 |
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