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| Status |
Public on Apr 29, 2020 |
| Title |
Microarray analysis of Polyoma Small T expressing U2OS cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In order to understand which pathways are involved and what genes are affected when small T is expressed in mammalian cells, we carried out microarray analaysis in these cell lines
Total cellulatr RNA was isolated at 20 hrs after doxycycline addition in samples 2,4 and 6 (+DOX), and was subjecyed to microarray analysis using Affymetrix Human Genome U133 Plus 2.0 (HG-U133_Plus_2) chips.
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| Overall design |
We had inducible Polyoma Small T cell lines. Docycyline was added to induce the expression of small T antigen. RNA was isolated from these cells and used for whole genome microarray analysis
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| Contributor(s) |
Andrabi S, Robert T |
| Citation(s) |
32404521, 34921839 |
| |
| Submission date |
Apr 28, 2020 |
| Last update date |
Feb 08, 2022 |
| Contact name |
Shaida Andrabi |
| E-mail(s) |
shaida.andrabi@uok.edu.in
|
| Organization name |
University of Kashmir
|
| Department |
Biochemistry
|
| Lab |
Cancer Biology and Cell Signaling
|
| Street address |
Srinagar
|
| City |
Srinagar |
| State/province |
Kashmir |
| ZIP/Postal code |
190006 |
| Country |
India |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA628984 |
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