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| Status |
Public on Jun 30, 2020 |
| Title |
Identification of genome-wide binding sites of Psi, Myc and Pol in Drosophila imaginal wing discs using Targeted DamID |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Targeted DamID was performed to identify binding targets of P-element somatic inhinitor (Psi), Myc and RNA Polymerase (using the RpII18 subunit common to all 3 polymerases). Scalloped(sd)-GAL4 was used to restrict expression to the wing disc.
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| Overall design |
Dam methylase fusion constructs under the control of UAS were expressed for 24 hours using the scalloped-GAL4 driver in the background of temperature-sensitive GAL80. 3 replicates each of Psi-Dam, Myc-Dam and Pol-Dam were normalised to the corresponding Dam-only control.
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| |
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| Contributor(s) |
Zaytseva O, Marshall OJ, Quinn LM |
| Citation(s) |
36692218 |
| |
| Submission date |
May 01, 2020 |
| Last update date |
May 12, 2023 |
| Contact name |
Olga Zaytseva |
| E-mail(s) |
olga.zaytseva@anu.edu.au
|
| Organization name |
John Curtin School of Medical Research
|
| Street address |
131 Garran Road
|
| City |
Canberra |
| State/province |
ACT |
| ZIP/Postal code |
2601 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE149721 |
Identification of genome-wide binding sites of Psi, Myc and Pol and gene expression following Psi or Myc knockdown in Drosophila larval wing tissue |
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| Relations |
| BioProject |
PRJNA629803 |
| SRA |
SRP259934 |
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