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| Status |
Public on Jun 30, 2020 |
| Title |
RNA-sequencing following Psi or Myc knockdown in Drosophila larval wing tissue |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNA-seq analysis of gene expression changes following the depletion of P-element somatic inhibitor (Psi) or Myc via RNAi in wing discs of mid-third instar larvae, compared with control. Differential splicing analysis was also performed on the Psi RNAi dataset.
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| Overall design |
Expression was performed using the tubulin-GAL4 driver in the background of temperature-sensitive GAL80, restricting RNAi knockdown to 3 days prior to collection. Larval wing discs were collected in lots of 20; 60 wings were pooled for each replicate before RNA extraction and sequencing. Samples were performed in triplicate; for analysis either Psi RNAi or Myc RNAi were compared to control
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| |
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| Contributor(s) |
Zaytseva O, Quinn LM, Evers M |
| Citation(s) |
36692218 |
| |
| Submission date |
May 01, 2020 |
| Last update date |
May 12, 2023 |
| Contact name |
Olga Zaytseva |
| E-mail(s) |
olga.zaytseva@anu.edu.au
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| Organization name |
John Curtin School of Medical Research
|
| Street address |
131 Garran Road
|
| City |
Canberra |
| State/province |
ACT |
| ZIP/Postal code |
2601 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE149721 |
Identification of genome-wide binding sites of Psi, Myc and Pol and gene expression following Psi or Myc knockdown in Drosophila larval wing tissue |
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| Relations |
| BioProject |
PRJNA629805 |
| SRA |
SRP259935 |
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