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| Status |
Public on Apr 28, 2022 |
| Title |
Global Analysis of Translational Reprogramming during Flagellar Regeneration Reveals the Role of Sphingolipid Metabolism in Ciliogenesis and Ciliopathies |
| Organisms |
Chlamydomonas reinhardtii; Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Protein synthesis is essential for cilium assembly, but little is known about translational reprogramming that occurs during ciliogenesis. Using ribosome footprinting, we perform global translatome profiling on Chlamydomonas during flagellar regeneration.
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| Overall design |
Chlamydomonas reinhardtii (strain CC-125) that were grown for 15, 30, 60, and 90 min after deflagellation to generate libraries for ribosome footprint sequencing (Ribo-seq) and RNA sequencing (RNA-seq) . A reference sample was collected from the same culture before deflagellation (Non-Treated). We also performed RNA sequencing for C.elegans mutants : sptl-1/sptl-2/sptl-3. Each strain was analyzed in biological duplicate.
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| Contributor(s) |
Wu D, Huang J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 04, 2020 |
| Last update date |
May 01, 2022 |
| Contact name |
Jingying Huang |
| E-mail(s) |
huang-jy18@mails.tsinghua.edu.cn
|
| Organization name |
Tsinghua University
|
| Department |
School of Life Sciences
|
| Street address |
Haidian District
|
| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (2) |
| GPL13657 |
Illumina HiSeq 2000 (Caenorhabditis elegans) |
| GPL15922 |
Illumina HiSeq 2000 (Chlamydomonas reinhardtii) |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA630259 |
| SRA |
SRP260039 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE149844_C_reinhardtii_allSamples_RNAseq_edgR_normalized_CPM.txt.gz |
273.0 Kb |
(ftp)(http) |
TXT |
| GSE149844_C_reinhardtii_allSamples_RNAseq_genes_exon_counts.txt.gz |
124.0 Kb |
(ftp)(http) |
TXT |
| GSE149844_C_reinhardtii_allSamples_Riboseq_edgR_normalized_CPM.txt.gz |
444.8 Kb |
(ftp)(http) |
TXT |
| GSE149844_C_reinhardtii_allSamples_Riboseq_genes_CDS_counts.txt.gz |
193.0 Kb |
(ftp)(http) |
TXT |
| GSE149844_RAW.tar |
319.1 Mb |
(http)(custom) |
TAR (of BW, TXT) |
| GSE149844_sptl_1_Expressed_gene_count_DEseq_normalized.txt.gz |
442.0 Kb |
(ftp)(http) |
TXT |
| GSE149844_sptl_2_Expressed_gene_count_DEseq_normalized.txt.gz |
520.7 Kb |
(ftp)(http) |
TXT |
| GSE149844_sptl_3_Expressed_gene_count_DEseq_normalized.txt.gz |
434.0 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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