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| Status |
Public on Mar 16, 2022 |
| Title |
Variability of bovine milk EV measurements in health and subclinical mastitis |
| Organism |
Bos taurus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Mastitis, the inflammation of the mammary gland, is one of the most prevalent diseases in dairy farming worldwide. Unfortunately, the disease is most often present in a subclinical type with no clear symptoms. The sooner the infection is detected, the less opportunities for the disease to progress and the more treatment options remain available. Milk microRNA (miRNA) encapsulated in extracellular vesicles (EV) have been proposed as potential biomarkers of different mammary gland conditions, including subclinical mastitis. However, little is known about the robustness of EV analysis regarding sampling time-point or natural infections. In order to estimate the reliability of EV measurements in raw bovine milk, we first evaluated the changes in EV size, concentration and miRNA cargo during three consecutive days. Then, we compared milk EV differences from natural infected quarters with high somatic cell count (SCC) with their healthy adjacent quarters with low SCC and quarters from uninfected udders. We found that milk EV miRNA cargo is very stable along three days and that infected quarters do not induce relevant changes in milk EV of adjacent healthy quarters, making them suitable controls. We observed cow-individual changes in immunoregulatory miRNA in quarters with chronic subclinical mastitis, pointing towards infection-specific alterations. Finally, we proposed bta-miR-223 as a potential indicator of subclinical mastitis prognosis in raw milk.
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| Overall design |
27 samples: 9 High Somatic Cell Count (High_SCC), 9 Low Somatic Cell Count (Low_SCC), 9 Healthy
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| Contributor(s) |
Saenz-de-Juano MD, Silvestrelli G, Bauersachs S, Ulbrich SE |
| Citation(s) |
35255807 |
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| Submission date |
May 04, 2020 |
| Last update date |
Mar 16, 2022 |
| Contact name |
Maria Desemparats Saenz-de-Juano |
| E-mail(s) |
mara.saenz@usys.ethz.ch
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| Organization name |
ETH Zurich
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| Street address |
Universitatstrasse 2
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| City |
Zurich |
| ZIP/Postal code |
8092 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL19172 |
Illumina HiSeq 2500 (Bos taurus) |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA630278 |
| SRA |
SRP260048 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE149856_processedfile_edgeR_Saenz-de-Juano.xlsx |
52.8 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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