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| Status |
Public on Sep 25, 2020 |
| Title |
Transcriptomic analysis of feline chronic gingivostomatitis (FCGS) |
| Organism |
Felis catus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The purpose of this study was to characterize the transcriptomic alterations accompanying the inflammation involved in feline chronic gingivostomatitis (FCGS). Towards this goal next-generation sequencing (NGS)-based gene expression profiling (RNA-Sequencing; RNA-Seq) was performed on matched pairs of FCGS diseased and healthy tissues obtained from three feline subjects.
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| Overall design |
A total of 6 total RNA samples were analyzed in this study. The study included matched pairs of inflamed feline chronic gingivostomatitis (FCGS; diseased) and adjacent healthy mucosal tissues from the area lateral to the palatoglossal folds from 3 individual feline subjects: Bart, Jerome, and Sadie. Full-thickness 6-8 mm biopsies were obtained from inflamed and adjacent healthy oral mucosa and were collected under general anesthesia. Tissues were snap-frozen in liquid nitrogen, total RNA was isolated, and then samples submitted for RNA-sequencing analysis.
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| Contributor(s) |
Borjesson DL, Vapniarsky-Arzi N, Tepper CG |
| Citation(s) |
32582783 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| P30 CA093373 |
Cancer Center Support Grant P30 |
UNIVERSITY OF CALIFORNIA DAVIS |
PRIMO N. LARA |
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| |
| Submission date |
May 05, 2020 |
| Last update date |
Sep 27, 2020 |
| Contact name |
Clifford G. Tepper |
| E-mail(s) |
cgtepper@ucdavis.edu
|
| Phone |
916-734-7195
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| Organization name |
UC Davis School of Medicine
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| Department |
Biochemistry and Molecular Medicine
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| Street address |
4645 2nd Avenue, Room 2300A
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| City |
Sacramento |
| State/province |
CA |
| ZIP/Postal code |
95817 |
| Country |
USA |
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| Platforms (1) |
| GPL26066 |
Illumina HiSeq 3000 (Felis catus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA630430 |
| SRA |
SRP260071 |
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