NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE150163 Query DataSets for GSE150163
Status Public on Feb 26, 2021
Title BRCA2 promotes DNA-RNA hybrid resolution by DDX5 helicase at DNA breaks to facilitate their repair
Organism Homo sapiens
Experiment type Other
Summary The BRCA2 tumor suppressor is a DNA double-strand break repair factor essential to maintain genome integrity. BRCA2-deficient cells spontaneously accumulate DNA-RNA hybrids, a known source of genome instability. However, the specific role of BRCA2 on these structures remains poorly understood. Here we identified the DEAD-box RNA helicase DDX5 as a BRCA2 interacting partner. We show that DDX5 associates with DNA-RNA hybrids that form in the vicinity of DSBs and this association is enhanced by BRCA2. Notably, BRCA2 stimulates the DNA-RNA hybrid helicase activity of DDX5. Impaired BRCA2-DDX5 interaction, as observed in cells bearing the breast cancer variant BRCA2-T207A, reduces DDX5 association with DSBs, decreases the number of RPA foci upon irradiation and impairs RAD51 repair foci formation. Our findings are consistent with DNA-RNA hybrids being an impediment for the repair of DSBs by homologous recombination and reveal BRCA2 and DDX5 as active players in their removal.
 
Overall design Genome-wide inspection of RNA-DNA hybrids accumulation in siC control and DDX5-depleted (siDDX5) K562 cells
 
Contributor(s) Sessa G, Gómez-González B, Silva S, Pérez-Calero C, Beaurepere R, Barroso S, Martineau S, Martin C, Ehlén Å, Martínez JS, Lombard B, Loew D, Vagner S, Aguilera A, Carreira A
Citation(s) 33634895
Submission date May 08, 2020
Last update date Mar 19, 2021
Contact name Andrés Aguilera
Organization name CABIMER, Universidad de Sevilla
Department Genome BIology
Lab Genome Instability & Cancer
Street address Av. Américo Vespucio 24
City Seville
ZIP/Postal code 41092
Country Spain
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (4)
GSM4525264 DRIPc_seq_siC_1 reanalysis
GSM4525265 DRIPc_seq_siDDX5_1
GSM4525266 DRIPc_seq_siC_2 reanalysis
Relations
BioProject PRJNA631281
SRA SRP260830

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE150163_RAW.tar 292.0 Mb (http)(custom) TAR (of BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.