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Status |
Public on Jun 18, 2020 |
Title |
Developmental Programming: Sheep Granulosa and Theca Cell-Specific Transcriptional Regulation by Prenatal Testosterone |
Organism |
Ovis aries |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Prenatal testosterone treated sheep, similar to PCOS women, manifest reduced cyclicity, functional hyperandrogenism and polycystic ovary (PCO) morphology. The PCO morphology results from increased follicular recruitment and persistence of antral follicles, consequence of reduced follicular growth and atresia, and driven by cell-specific gene expression changes that are poorly understood. Therefore, utilizing RNA sequencing, cell-specific transcriptional changes were assessed in laser capture microdissection isolated antral follicular granulosa and theca cells from 21 months-of- age control and prenatal testosterone treated sheep.
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Overall design |
5 control sheep and 5 prenatal testosterone treated sheep, each with a theca cell and granulosa cell sample
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Contributor(s) |
Puttabyatappa M, Dou J, Guo X, Dumesic D, Bakulski KM, Padmanabhan V |
Citation(s) |
- Puttabyatappa M, Guo X, Dou J, Dumesic D et al. Developmental Programming: Sheep Granulosa and Theca Cell-Specific Transcriptional Regulation by Prenatal Testosterone. Endocrinology 2020 Aug 1;161(8). PMID: 32516392
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Submission date |
May 11, 2020 |
Last update date |
Jul 30, 2020 |
Contact name |
Kelly Bakulski |
E-mail(s) |
bakulski@umich.edu
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Organization name |
University of Michigan
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Department |
Department of Epidemiology
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Street address |
1415 Washington Heights
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City |
Ann Arbor |
State/province |
MI |
ZIP/Postal code |
48109 |
Country |
USA |
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Platforms (1) |
GPL27721 |
Illumina NovaSeq 6000 (Ovis aries) |
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Samples (20)
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Relations |
BioProject |
PRJNA631614 |
SRA |
SRP261061 |