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| Status |
Public on Dec 07, 2020 |
| Title |
Transcriptomic differences between mouse tumor-infiltrating and splenic regulatory T cells with or without Treg-specific loss of CD177 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of this study was to identify transcriptomic differences between splenic regulatory T cells and tumor-infiltrating regualtory T cells with or without Treg-specific loss of CD177.
Methods: MC38 tumors from CD177flox/flox/FOXP3-CreYFP and CD177flox/flox C57Bl/6 mice were ezymatically dissocaited and a single cell suspension was obtained. Spleens from the same mice were collected and single cell suspension was obtained by mechanical dissociation. Regulatory T cells, identified as CD45+ CD4+ CD25hi GITR+, were isolated by flourenscence activated cell sorting. RNA from the regualtory T cell populations were isolated and libraries were generated using the NextSeq500 system. Samples were aligned using Kallisto pseudoalignment protocol and the mm19 mouse genome. Counts were processed and aggregated by gene symbol using the sleuth R package.
Results: The pro-survival gene Bcl2l1 is selectivley upregulated in tumor-infiltratign regulatory T cells compared to splenic T cells
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| Overall design |
Analyze differentially regulated genes in tumor-infiltrating regualtory T cells compared to splenic regulatory T cells in wild-type or Treg-specific CD177 knockout mice
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| Contributor(s) |
Zhang W, Zhou D, Kolb R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 12, 2020 |
| Last update date |
Dec 07, 2020 |
| Contact name |
Ryan Kolb |
| E-mail(s) |
ryankolb@ufl.edu
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| Organization name |
University of Florida
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| Department |
Pathology, Immunology and Laboratory Medicine
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| Street address |
1275 Center Drive, M656
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| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA632003 |
| SRA |
SRP261323 |
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