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Series GSE150504 Query DataSets for GSE150504
Status Public on Mar 18, 2021
Title FGFR2 fusion protein-driven mouse models of intrahepatic cholangiocarcinoma unveil a necessary role for Erk signaling
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Four structurally different FFs were expressed in Tp53-/- mouse liver organoids. The tumorigenic properties of genetically-modified liver organoids were assessed by intrahepatic or subcutaneous transplantation in immuno-deficient mice. RNA was extracted from tumors and subjected to RNAseq. Transcriptional profiling identified up/down-regulated signatures shared between human and mouse cholangiocarcinomas driven by FGFR2 fusion proteins.
 
Overall design Tumors diagnosed as cholangiocarcinoma, obtained upon s.c. or intra-hepatic transplantation of Tp53 null liver organoids engineered to express either FGFR2-BICC1 or FGFR2-TACC3, were excised and subjected to RNAseq. RNAseq data were analyzed in order to discover transcriptional similarities between human and mouse cholangiocarcinomas driven by oncogenic FGFR2 fusion proteins.
 
Contributor(s) Forcato M, Cristinziano G, Segatto O, Cristofoletti C
Citation(s) 33741397
Submission date May 13, 2020
Last update date Mar 21, 2021
Contact name Mattia Forcato
Organization name University of Padova
Street address Via Ugo Bassi, 58/B
City Padova
ZIP/Postal code 35131
Country Italy
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (14)
GSM4551522 Liver_p53_het
GSM4551523 Liver_p53_homo
GSM4551524 Tumor_Tacc3_intra_rep1
Relations
BioProject PRJNA632601
SRA SRP261489

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE150504_RNAseq_expressionMatrix_counts.txt.gz 503.5 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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