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Status |
Public on Mar 18, 2021 |
Title |
FGFR2 fusion protein-driven mouse models of intrahepatic cholangiocarcinoma unveil a necessary role for Erk signaling |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Four structurally different FFs were expressed in Tp53-/- mouse liver organoids. The tumorigenic properties of genetically-modified liver organoids were assessed by intrahepatic or subcutaneous transplantation in immuno-deficient mice. RNA was extracted from tumors and subjected to RNAseq. Transcriptional profiling identified up/down-regulated signatures shared between human and mouse cholangiocarcinomas driven by FGFR2 fusion proteins.
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Overall design |
Tumors diagnosed as cholangiocarcinoma, obtained upon s.c. or intra-hepatic transplantation of Tp53 null liver organoids engineered to express either FGFR2-BICC1 or FGFR2-TACC3, were excised and subjected to RNAseq. RNAseq data were analyzed in order to discover transcriptional similarities between human and mouse cholangiocarcinomas driven by oncogenic FGFR2 fusion proteins.
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Contributor(s) |
Forcato M, Cristinziano G, Segatto O, Cristofoletti C |
Citation(s) |
33741397 |
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Submission date |
May 13, 2020 |
Last update date |
Mar 21, 2021 |
Contact name |
Mattia Forcato |
Organization name |
University of Padova
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Street address |
Via Ugo Bassi, 58/B
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City |
Padova |
ZIP/Postal code |
35131 |
Country |
Italy |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (14)
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Relations |
BioProject |
PRJNA632601 |
SRA |
SRP261489 |