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| Status |
Public on Jul 01, 2020 |
| Title |
RNA sequencing of murine conventional dendritic cell lineage 1 (cDC1) cells from mesenteric lymph nodes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
A gene expression profile of the conventional dendritic cell lineage 1 from murine mesenteric lymph nodes (MLN) were constructed based on RNA-seq from nine biological biopsies. The gene expression profile was afterwards used to infer (in silico) a cDC1-specific protein-protein interactome.
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| Overall design |
Nine mesenteric lymph nodes were taken from healthy/untreated female 7-10 weeks old C57BL6 mice and CD11c+MHCIIhiXCR-1+CD103+ cDC1 cells were flow cytometry cell sorted. 50bp single-read mRNA sequencing was performed on the cDC1 cells from nine MLNs on illumina machines, resulting in nine FASTQ samples.
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| Contributor(s) |
Niss K, Gomez-Casado C, Hjaltelin JX, Joeris T, Agace WW, Belling KG, Brunak S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
May 14, 2020 |
| Last update date |
Jul 03, 2020 |
| Contact name |
Søren Brunak |
| E-mail(s) |
soren.brunak@cpr.ku.dk
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| Organization name |
University of Copenhagen
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| Department |
Novo Nordisk Foundation Center for Protein Research
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| Street address |
Blegdamsvej 3B
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| City |
Copenhagen |
| ZIP/Postal code |
2100 |
| Country |
Denmark |
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| Platforms (1) |
| GPL15103 |
Illumina HiSeq 1000 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA632752 |
| SRA |
SRP261581 |
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