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| Status |
Public on Sep 08, 2022 |
| Title |
Gene expression profile of primary CLL cells transduced with a mutated form on NOTCH1 following IgM stimulation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In CLL, NOTCH1 is the most commonly mutated gene at diagnosis and it is associated with a poor outcome. The mechanisms underlying how NOTCH1 contributes to disease progression, resistance to treatment and Richter’s transformation remain largely unknown. Data suggests a link between BCR activation and NOTCH1 signalling so the main goal of this study is to compare the gene expression profile of primary CLL cells transduced with the intracellular part of NOTCH1 lacking of the PEST domain vs cells transduced with an empty vector following IgM stimulation.
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| Overall design |
Primary CLL cells were isolated through cell sorting 4 days post transusction with a NOTCH1ΔPEST or an empty retroviral vector. The cells were then stimulated with a F(ab')2 Anti-human IgM for 48 hours
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| Contributor(s) |
Mangolini M, Adam G, Mohorianu I, Ringshausen I |
| Citation(s) |
36266281 |
| |
| Submission date |
May 14, 2020 |
| Last update date |
Oct 31, 2022 |
| Contact name |
Irina Mohorianu |
| E-mail(s) |
data-submissions@stemcells.cam.ac.uk
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| Organization name |
University of Cambridge
|
| Department |
Wellcome-MRC Cambridge Stem Cell Institute
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| Street address |
Puddicombe Way
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| City |
Cambridge |
| ZIP/Postal code |
CB2 0AW |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (20)
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| This SubSeries is part of SuperSeries: |
| GSE150610 |
Gene expression profile of primary CLL cells transduced with a mutated NOTCH1 |
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| Relations |
| BioProject |
PRJNA632901 |
| SRA |
SRP261674 |
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