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Series GSE150595 Query DataSets for GSE150595
Status Public on Sep 08, 2022
Title Gene expression profile of primary CLL cells transduced with a mutated form on NOTCH1 following IgM stimulation
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In CLL, NOTCH1 is the most commonly mutated gene at diagnosis and it is associated with a poor outcome. The mechanisms underlying how NOTCH1 contributes to disease progression, resistance to treatment and Richter’s transformation remain largely unknown. Data suggests a link between BCR activation and NOTCH1 signalling so the main goal of this study is to compare the gene expression profile of primary CLL cells transduced with the intracellular part of NOTCH1 lacking of the PEST domain vs cells transduced with an empty vector following IgM stimulation.
Overall design Primary CLL cells were isolated through cell sorting 4 days post transusction with a NOTCH1ΔPEST or an empty retroviral vector. The cells were then stimulated with a F(ab')2 Anti-human IgM for 48 hours
Contributor(s) Mangolini M, Adam G, Mohorianu I, Ringshausen I
Citation(s) 36266281
Submission date May 14, 2020
Last update date Oct 31, 2022
Contact name Irina Mohorianu
Organization name University of Cambridge
Department Wellcome-MRC Cambridge Stem Cell Institute
Street address Puddicombe Way
City Cambridge
ZIP/Postal code CB2 0AW
Country United Kingdom
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (20)
GSM4552930 P1_CTR
GSM4552931 P1_CTR_IGM
GSM4552932 P1_NOTCH
This SubSeries is part of SuperSeries:
GSE150610 Gene expression profile of primary CLL cells transduced with a mutated NOTCH1
BioProject PRJNA632901
SRA SRP261674

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Supplementary file Size Download File type/resource
GSE150595_normalised_expressions.csv.gz 2.5 Mb (ftp)(http) CSV
GSE150595_raw_expressions.csv.gz 837.5 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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