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Status |
Public on May 19, 2020 |
Title |
Varenicline reduces ethanol consumption in male C57BL/6J mice: Potential gene expression mechanisms |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
In recent years, varenicline has been shown to decrease ethanol consumption in adult rodents providing support as a therapeutic treatment for alcohol use disorder (AUD). Varenicline is a partial agonist at alpha4beta2 nicotinic acetylcholine receptors, but interacts with other receptors at high concentrations. In order to gain a greater understanding of the mechanisms by which varenicline decreases ethanol consumption, we performed RNA sequencing on striatal tissue from C57BL/6J mice treated with varenicline or saline prior to an ethanol Drinking-in-the-Dark (DID) session. Using classical tools to analyze RNA sequencing data, we found 809 differentially expressed genes with Cuffdiff and one significant co-expression network using WGCNA.
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Overall design |
Striatal gene expression from animals treated with saline or 2 mg/kg varenicline prior to ethanol Drinking-in-the-Dark
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Contributor(s) |
Kamens HM, Dziak JJ, Horton WJ, Silva CP, Sebastian A, Albert I, Li R |
Citation missing |
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Submission date |
May 18, 2020 |
Last update date |
May 19, 2020 |
Contact name |
Aswathy Sebastian |
E-mail(s) |
azs13@psu.edu
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Phone |
5204613475
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Organization name |
PennState
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Street address |
University Park
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City |
State College |
ZIP/Postal code |
16801 |
Country |
USA |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (12)
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Relations |
BioProject |
PRJNA633622 |
SRA |
SRP262213 |