|
| Status |
Public on Aug 11, 2021 |
| Title |
Identification of nucleolar associated domains (NADS) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
This study aims to identify genomic contacts with the nucleolus (nucleolar associated domains, NADs). We established a novel methodology that we applied to identify NADs in embryonic stem cells (ESCs). The results revealed unprecedented distinct layers of genome compartmentalization that are distinguished according to gene expression and chromatin states thereby providing novel insights into basic principles of genome organization and its role in gene expression and cell function.
|
| |
|
| Overall design |
Nucleolar-DamID, ChIP seq, RNA seq in mouse ESC
|
| |
|
| Contributor(s) |
Bersaglieri C, Kresoja-Rakic J, Gupta S, Bär D, Kuzyakiv R, Santoro R |
| Citation(s) |
35304483 |
|
Cristiana Bersaglieri, Jelena Kresoja-Rakic, Shivani Gupta, Dominik Bär, Rostyslav Kuzyakiv, and Raffaella Santoro. Genome-wide maps of nucleolus interactions reveal distinct layers of repressive chromatin domains. bioRxiv 2020.11.17.386797. doi:10.1101/2020.11.17.386797
|
| |
| Submission date |
May 19, 2020 |
| Last update date |
Apr 06, 2022 |
| Contact name |
Raffaella Santoro |
| E-mail(s) |
raffaella.santoro@dmmd.uzh.ch
|
| Phone |
+41 44 635 54 75
|
| Organization name |
University of Zurich
|
| Department |
Dep. of Molecular Mechanisms of Disease
|
| Street address |
Winterthurerstrasse 190
|
| City |
Zurich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
| |
|
| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA633792 |
| SRA |
SRP262289 |
Less...
More...