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Status |
Public on Jun 01, 2020 |
Title |
Candida albicans sexual regulators control expression of genes important for commensal fitness [RNA-seq] |
Organisms |
Candida albicans; Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We found through competitive fitness experiments that transcription factors that control the mating regulatory program of C. albicans also control the ability of this fungus to colonize the gastrointestinal tract of mice as a commensal. To determine the role of these TFs, we performed mRNA-seq on luminal contents of C. albicans-colonized mice to determine overall expression patterns that could be correlated to the fitness phenotypes of the mutants in vivo.
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Overall design |
Mice were gastrointestinally-colonized with one of seven C. albicans strains, WT (ySN250 or ySN425), hyperfit mutants WOR1-OE GUT, efg1, or efg1wor1, or hypofit mutants wor1 or czf1. After 10 days of colonization, mice were sacrificed and luminal contents isolated from the large intestines. mRNA-seq using standard Illumina kits was performed.
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Contributor(s) |
Witchley JN, Noble SM |
Citation missing |
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Submission date |
May 19, 2020 |
Last update date |
Jun 01, 2020 |
Contact name |
Suzanne M Noble |
E-mail(s) |
suzanne.noble@ucsf.edu
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Organization name |
UCSF
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Street address |
513 Parnassus Ave., Box 0414
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City |
San Francisco |
State/province |
CA |
ZIP/Postal code |
94143-0414 |
Country |
USA |
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Platforms (2) |
GPL24129 |
Illumina HiSeq 4000 (Candida albicans) |
GPL25966 |
Illumina HiSeq 4000 (Candida albicans; Mus musculus) |
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Samples (58)
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This SubSeries is part of SuperSeries: |
GSE150879 |
Recording of DNA binding events during gut commensalism reveals the action of a repurposed Candida albicans regulatory network |
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Relations |
BioProject |
PRJNA633862 |
SRA |
SRP262333 |