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Series GSE150860 Query DataSets for GSE150860
Status Public on Jun 28, 2021
Title A role for tRNA modifications in the expansion of proteome diversity
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The mechanisms that drive the evolution of proteome diversity are poorly understood. Here we investigate the functional significance of eight eukaryotic tRNAs that contain inosine in the wobble position of the anticodon (I34-tRNAs). We find that these modified tRNAs are essential to translate a subset of the eukaryotic proteome composed of eukaryote-specific, low-complexity polypeptides involved in interactions with the extracellular environment, including proteins containing mucin-like domains. Depletion of I34-tRNAs in human cells leads to impaired translation of these proteins, and morphological and cell adhesion phenotypes. We further show that translation defects are strictly dependent on codon distribution and composition of transcripts. Genes coding for the precursors of I34-tRNAs are almost absent in prokaryotes and abundant in eukaryotes. We conclude that I34-tRNAs bestowed eukaryotes with the ability to expand their proteomes with a new set of highly-biased genetic sequences that provided these organisms with novel extracellular functionalities.
 
Overall design RNA-Seq obtained from Total RNA and from high-polysomal fractions of HEK293T cells Control and HEK293T (CRISPR/Cas9-mediated) ADAT2 KD cells
 
Contributor(s) Torres AG, Reina García O, Ribas de Pouplana L, Novoa EM
Citation(s) 34125917
Submission date May 19, 2020
Last update date Jun 28, 2021
Contact name Oscar Reina Garcia
E-mail(s) oscar.reina@irbbarcelona.org
Organization name IRB Barcelona
Department Biostatistics and Bioinformatics
Street address C/Baldiri Reixac 10
City Barcelona
State/province Barcelona
ZIP/Postal code 08028
Country Spain
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (48)
GSM4559811 KO hightranslRNA Replicate 1 Lane 1
GSM4559812 KO hightranslRNA Replicate 1 Lane 2
GSM4559813 KO hightranslRNA Replicate 1 Lane 3
Relations
BioProject PRJNA633874
SRA SRP262339

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE150860_Rsubread_1.28.1_featureCounts_raw_entrez_ncbi_hg38_atorres_2020.xlsx 6.0 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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