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Series GSE151049 Query DataSets for GSE151049
Status Public on Dec 05, 2020
Title Osteocyte necrosis triggers osteoclast-mediated bone loss through macrophage-inducible C-type lectin
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary It is generally believed that bone micro-fractures, associated with osteocyte death, lead to osteoclast recruitment and thereupon to removal and replacement of damaged bone. However, the underlying mechanism is still incompletely defined. In this study we hypothesized that the pattern recognition receptor macrophage-inducible C-type lectin (Mincle) is expressed in osteoclasts and is responsible for the recognition of osteocyte death. To understand the mechanistic role of Mincle in osteoclasts, we performed in-depth analysis of the effect of Mincle deficiency on the genomic transcriptional network of osteoclasts. Whole transcriptome RNA sequencing (RNAseq) was performed on osteoclasts stimulated with necrotic osteocyte supernatant compared to control osteoclasts (stimulated with viable osteocyte supernatant), derived from either wildtype or Mincle knockout mice.
 
Overall design In vitro differentiated osteoclasts from wildtype (WT, C57BL/6 mice) or Mincle knockout (KO, C57BL/6 background) mice were stimulated on day 1 of culture with either supernatant from viable osteocytes (control condition) or supernatant from necrotic osteocytes (generated by serum starvation) for 24h. The osteoclast RNA was extracted on day 3 of differentiation for RNA-seq.
 
Contributor(s) Bozec A, Andreev D
Citation(s) 32773408
Submission date May 22, 2020
Last update date May 21, 2022
Contact name Darja Andreev
E-mail(s) darja-andreev@gmx.de
Organization name Friedrich-Alexander-University Erlangen-Nürnberg and Universitätsklinikum Erlangen
Department Medicine 3
Lab AG Bozec
Street address Glückstraße 6
City Erlangen
ZIP/Postal code 91054
Country Germany
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (12)
GSM4565362 Ct1
GSM4565363 Ct2
GSM4565364 Ct3
Relations
BioProject PRJNA634512
SRA SRP262935

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE151049_genes.readcount.xls.gz 527.3 Kb (ftp)(http) XLS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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