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Status |
Public on Sep 16, 2020 |
Title |
Plasticity of ether lipids promotes ferroptosis susceptibility and evasion |
Organism |
Homo sapiens |
Experiment type |
Other
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Summary |
Ferroptosis, an iron-dependent, non-apoptotic cell death program, is involved in a variety of degenerative diseases and represents a targetable vulnerability in certain cancers. The ferroptosis-susceptible cell-state can either preexist in cells arising from certain lineages or be acquired during cell-state transitions. Precisely how ferroptosis susceptibility is dynamically regulated remains poorly understood. Using genome-wide CRISPR/Cas9 suppressor screens, we identify a key role for peroxisome–ether phospholipid genes in driving ferroptosis susceptibility and evasion in ovarian cancer.
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Overall design |
Ovcar8 CRISPR library infected cells treated with escalating doses of RSL3 (Selleckchem) for 4 days each: 0.5 μM, 1 μM, and 2μM. 1×10^7 cells each were collected from the surviving population of RSL3-treated cells and an endpoint-matched untreated population for downstream sequencing analysis.
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Contributor(s) |
Henry WS, Keys HR |
Citation missing |
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Submission date |
May 22, 2020 |
Last update date |
Sep 18, 2020 |
Contact name |
Yilong Zou |
E-mail(s) |
zouyilong@westlake.edu.cn
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Organization name |
Westlake University
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Department |
School of Life Sciences
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Lab |
Laboratory of Functional Lipidomics and Metabolic Regulation
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Street address |
Shilongshan Road #18, Cloud Town, Xihu District
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City |
Hangzhou |
State/province |
Zhejiang |
ZIP/Postal code |
310024 |
Country |
China |
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Platforms (1) |
GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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Samples (6)
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Relations |
BioProject |
PRJNA634531 |
SRA |
SRP262968 |