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Series GSE151062 Query DataSets for GSE151062
Status Public on Sep 16, 2020
Title Plasticity of ether lipids promotes ferroptosis susceptibility and evasion
Organism Homo sapiens
Experiment type Other
Summary Ferroptosis, an iron-dependent, non-apoptotic cell death program, is involved in a variety of degenerative diseases and represents a targetable vulnerability in certain cancers. The ferroptosis-susceptible cell-state can either preexist in cells arising from certain lineages or be acquired during cell-state transitions. Precisely how ferroptosis susceptibility is dynamically regulated remains poorly understood. Using genome-wide CRISPR/Cas9 suppressor screens, we identify a key role for peroxisome–ether phospholipid genes in driving ferroptosis susceptibility and evasion in ovarian cancer.
 
Overall design Ovcar8 CRISPR library infected cells treated with escalating doses of RSL3 (Selleckchem) for 4 days each: 0.5 μM, 1 μM, and 2μM. 1×10^7 cells each were collected from the surviving population of RSL3-treated cells and an endpoint-matched untreated population for downstream sequencing analysis.
 
Contributor(s) Henry WS, Keys HR
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Submission date May 22, 2020
Last update date Sep 18, 2020
Contact name Yilong Zou
E-mail(s) zouyilong@westlake.edu.cn
Organization name Westlake University
Department School of Life Sciences
Lab Laboratory of Functional Lipidomics and Metabolic Regulation
Street address Shilongshan Road #18, Cloud Town, Xihu District
City Hangzhou
State/province Zhejiang
ZIP/Postal code 310024
Country China
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (6)
GSM4565569 Untreated_OVCAR8_Day12
GSM4565570 RSL3_OVCAR8_Day12
GSM4565571 Untreated_OVCAR8_Day8
Relations
BioProject PRJNA634531
SRA SRP262968

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE151062_RAW.tar 4.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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