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Series GSE151067 Query DataSets for GSE151067
Status Public on Jun 17, 2020
Title Multisite methylation profiling of primary human meningioma
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary Multiple stereotatically separate sites from human meningioma were processed for methlyation profiling
Meningiomas are the most common primary intracranial tumors, but the molecular drivers of meningioma tumorigenesis are poorly understood. We hypothesized that investigating intratumor heterogeneity in meningiomas would elucidate biologic drivers and reveal new targets for molecular therapy. To test this hypothesis, we performed multiplatform molecular profiling of 86 spatially-distinct samples from 13 human meningiomas. Our data reveal that regional alterations in chromosome structure underlie clonal transcriptomic, epigenomic, and histopathologic signatures in meningioma. Stereotactic co-registration of sample coordinates to preoperative magnetic resonance images further demonstrated that high apparent diffusion coefficient (ADC) distinguished meningioma regions with proliferating cells enriched for developmental gene expression programs. To understand the function of these genes in meningioma, we developed a human cerebral organoid model of meningioma and validated the high ADC marker genes CDH2 and PTPRZ1 as potential targets for meningioma therapy using live imaging, single cell RNA sequencing, CRISPR interference, and pharmacology.
 
Overall design A total of 88 spatially-distinct samples from 13 human meningiomas were profiled
 
Contributor(s) Vasudevan HN, Raleigh DR
Citation(s) 32517746, 32968068, 35759011
Submission date May 22, 2020
Last update date Nov 17, 2022
Contact name Harish Vasudevan
Organization name University of California San Francisco
Department Radiation Oncology
Street address 555 Mission Bay Blvd South, Room 452H
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platforms (1)
GPL23976 Illumina Infinium HumanMethylation850 BeadChip
Samples (88)
GSM4565721 201959750116_R01C01
GSM4565722 201959750116_R02C01
GSM4565723 201959750116_R03C01
Relations
BioProject PRJNA634543

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE151067_BetaValues_MeningiomaMethylation_Run1.txt.gz 244.2 Mb (ftp)(http) TXT
GSE151067_BetaValues_MeningiomaMethylation_Run2.txt.gz 298.6 Mb (ftp)(http) TXT
GSE151067_BetaValues_MeningiomaMethylation_Run3.txt.gz 9.9 Mb (ftp)(http) TXT
GSE151067_RAW.tar 1.2 Gb (http)(custom) TAR (of IDAT)
Processed data are available on Series record

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