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Status |
Public on Jun 17, 2020 |
Title |
Multisite methylation profiling of primary human meningioma |
Organism |
Homo sapiens |
Experiment type |
Methylation profiling by genome tiling array
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Summary |
Multiple stereotatically separate sites from human meningioma were processed for methlyation profiling Meningiomas are the most common primary intracranial tumors, but the molecular drivers of meningioma tumorigenesis are poorly understood. We hypothesized that investigating intratumor heterogeneity in meningiomas would elucidate biologic drivers and reveal new targets for molecular therapy. To test this hypothesis, we performed multiplatform molecular profiling of 86 spatially-distinct samples from 13 human meningiomas. Our data reveal that regional alterations in chromosome structure underlie clonal transcriptomic, epigenomic, and histopathologic signatures in meningioma. Stereotactic co-registration of sample coordinates to preoperative magnetic resonance images further demonstrated that high apparent diffusion coefficient (ADC) distinguished meningioma regions with proliferating cells enriched for developmental gene expression programs. To understand the function of these genes in meningioma, we developed a human cerebral organoid model of meningioma and validated the high ADC marker genes CDH2 and PTPRZ1 as potential targets for meningioma therapy using live imaging, single cell RNA sequencing, CRISPR interference, and pharmacology.
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Overall design |
A total of 88 spatially-distinct samples from 13 human meningiomas were profiled
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Contributor(s) |
Vasudevan HN, Raleigh DR |
Citation(s) |
32517746, 32968068, 35759011 |
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Submission date |
May 22, 2020 |
Last update date |
Nov 17, 2022 |
Contact name |
Harish Vasudevan |
Organization name |
University of California San Francisco
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Department |
Radiation Oncology
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Street address |
555 Mission Bay Blvd South, Room 452H
|
City |
San Francisco |
State/province |
CA |
ZIP/Postal code |
94158 |
Country |
USA |
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Platforms (1) |
GPL23976 |
Illumina Infinium HumanMethylation850 BeadChip |
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Samples (88)
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Relations |
BioProject |
PRJNA634543 |