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| Status |
Public on Jul 16, 2021 |
| Title |
Generation of ovarian follicles from mouse pluripotent stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Oocytes mature in a specialized fluid-filled sac, the ovarian follicle, which provides signals needed for meiosis and germ cell growth. Methods have been developed to generate functional oocytes from pluripotent stem cell–derived primordial germ cell–like cells (PGCLCs) when placed in culture with embryonic ovarian somatic cells. In this study, we developed culture conditions to recreate the stepwise differentiation process from pluripotent cells to fetal ovarian somatic cell–like cells (FOSLCs). When FOSLCs were aggregated with PGCLCs derived from mouse embryonic stem cells, the PGCLCs entered meiosis to generate functional oocytes capable of fertilization and development to live offspring. Generating functional mouse oocytes in a reconstituted ovarian environment provides a method for in vitro oocyte production and follicle generation for a better understanding of mammalian reproduction.
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| |
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| Overall design |
Examination of single-cell transcriptomes in the in vivo gonad somatic cells and the in vitro induced ES cell-derived gonad smatic cells.
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| |
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| Contributor(s) |
Suzuki T, Hayashi K, Yoshino T |
| Citation(s) |
34437124 |
| |
| Submission date |
May 25, 2020 |
| Last update date |
Sep 08, 2021 |
| Contact name |
Takahiro Suzuki |
| E-mail(s) |
takahiro.suzuki.aa@riken.jp
|
| Phone |
81-45-503-9222
|
| Organization name |
RIKEN
|
| Department |
Center for Integrative Medical Sciences
|
| Lab |
Laboratory for Cellular Function Conversion Technologies
|
| Street address |
1-7-22 Suehiro-cho, Tsurumi-ku
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA634880 |
| SRA |
SRP263465 |
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