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Series GSE151217 Query DataSets for GSE151217
Status Public on Dec 22, 2021
Title The effect of S309W mutation on SP7 genomic binding in chondrocytes
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary SP7/Osterix is a transcription factor critical for osteoblast maturation and bone formation. We identidied a missense variant (c.926C>G:p.S309W) in SP7 in a patient with a unique high turnover bone disease. Mice with the corresponding variant similarly showed a complex skeletal phenotype distinct from that of Sp7-null mice. We therefore performed ChIP-Seq in primary chondrocytes to study how the mutation alters the genomic binding of SP7.
 
Overall design Primary mouse chondrocytes isolated from proximal tibia and distal femur of 7 day old male mice cardiomyocytes were cultured in DMEM/F12 media supplemented with 10% FBS, P/S, and 50 μg/mL ascorbic acid. Retrovirus expressing either wild-type SP7 or S309W variant of SP7 were produced with HEK-derived ECO cells and used for infecting primary chondrocytes. About 5 days post-infection, chondrocytes were harvested and cross-linked with 1% formaldehyde for genomic DNA isolation.
 
Contributor(s) Lui JC, Baron J, Hojo H
Citation(s) 35121733
Submission date May 26, 2020
Last update date Feb 23, 2022
Contact name Hironori Hojo
E-mail(s) hojo@tetrapod.t.u-tokyo.ac.jp
Phone +81-3-5841-1427
Organization name The University of Tokyo
Department Center for Disease Biology and Integrative Medicine
Lab Clinical Biotechnology
Street address 7-3-1 Hongo
City Bunkyo-ku
State/province Tokyo
ZIP/Postal code 113-8655
Country Japan
 
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (3)
GSM4568704 Primary chondrocytes - input DNA
GSM4568705 Primary chondrocytes - wild type FLAG-SP7
GSM4568706 Primary chondrocytes - mutant FLAG-SP7
Relations
BioProject PRJNA635142
SRA SRP264822

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Supplementary file Size Download File type/resource
GSE151217_RAW.tar 360.0 Kb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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