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Series GSE151263 Query DataSets for GSE151263
Status Public on Jul 10, 2020
Title Single Cell RNA Sequencing Identifies an Early Monocyte Gene Signature in Acute Respiratory Distress Syndrome
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The acute respiratory distress syndrome (ARDS) results from overwhelming pulmonary inflammation. We used single cell RNA sequencing to probe ARDS at a higher resolution for insights into ARDS pathogenesis. Peripheral blood mononuclear cells of patients with pneumonia and sepsis with early ARDS were compared to that of sepsis patients who did not develop ARDS. Monocyte clusters from ARDS patients revealed multiple distinguishing characteristics in comparison to monocytes from patients without ARDS including down-regulation of SOCS3 expression accompanied by a pro-inflammatory signature with up-regulation of multiple type I IFN-induced genes, especially in CD16+ cells. These data show that monocytes of ARDS patients up-regulate expression of genes not just restricted to those associated with inflammation. Together, our findings identify molecules that are likely involved in ARDS pathogenesis that may inform biomarker and therapeutic development.
 
Overall design 7 Frozen PBMC samples, 4 with sepsis only, 3 with sepsis and ARDS
 
Contributor(s) Ray P, Ray A, Chen J, Rosborough BR, Chen W
Citation(s)
  • Jiang Y, Rosborough BR, Chen J, Das S et al. Single cell RNA sequencing identifies an early monocyte gene signature in acute respiratory distress syndrome. JCI Insight 2020 Jul 9;5(13). PMID: 32554932
Submission date May 27, 2020
Last update date Jul 10, 2020
Contact name Anuradha Ray
Organization name University of Pittsburgh
Department Medicine/Pulmonary
Lab Ray
Street address 3459 Fifth Ave, MUH 628 NW
City Pittsburgh
State/province PA
ZIP/Postal code 15213
Country USA
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (7)
GSM4569780 PBMC from patient 1 sepsis+ARDS
GSM4569781 PBMC from patient 2 sepsis+ARDS
GSM4569782 PBMC from patient 3 sepsis+ARDS
Relations
BioProject PRJNA635353
SRA SRP264928

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE151263_RAW.tar 29.7 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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