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Status |
Public on Jul 10, 2020 |
Title |
Single Cell RNA Sequencing Identifies an Early Monocyte Gene Signature in Acute Respiratory Distress Syndrome |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The acute respiratory distress syndrome (ARDS) results from overwhelming pulmonary inflammation. We used single cell RNA sequencing to probe ARDS at a higher resolution for insights into ARDS pathogenesis. Peripheral blood mononuclear cells of patients with pneumonia and sepsis with early ARDS were compared to that of sepsis patients who did not develop ARDS. Monocyte clusters from ARDS patients revealed multiple distinguishing characteristics in comparison to monocytes from patients without ARDS including down-regulation of SOCS3 expression accompanied by a pro-inflammatory signature with up-regulation of multiple type I IFN-induced genes, especially in CD16+ cells. These data show that monocytes of ARDS patients up-regulate expression of genes not just restricted to those associated with inflammation. Together, our findings identify molecules that are likely involved in ARDS pathogenesis that may inform biomarker and therapeutic development.
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Overall design |
7 Frozen PBMC samples, 4 with sepsis only, 3 with sepsis and ARDS
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Contributor(s) |
Ray P, Ray A, Chen J, Rosborough BR, Chen W |
Citation(s) |
- Jiang Y, Rosborough BR, Chen J, Das S et al. Single cell RNA sequencing identifies an early monocyte gene signature in acute respiratory distress syndrome. JCI Insight 2020 Jul 9;5(13). PMID: 32554932
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Submission date |
May 27, 2020 |
Last update date |
Jul 10, 2020 |
Contact name |
Anuradha Ray |
Organization name |
University of Pittsburgh
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Department |
Medicine/Pulmonary
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Lab |
Ray
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Street address |
3459 Fifth Ave, MUH 628 NW
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City |
Pittsburgh |
State/province |
PA |
ZIP/Postal code |
15213 |
Country |
USA |
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Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (7)
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Relations |
BioProject |
PRJNA635353 |
SRA |
SRP264928 |