NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE151363 Query DataSets for GSE151363
Status Public on Jun 01, 2021
Title The TFIID subunit TAF4 is required for pancreatic beta cell function and identity [ATAC-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We selectively inactivated the Taf4 subunit of general transcription factor TFIID in adult murine pancreatic beta cells (BCs). Taf4 inactivation rapidly diminishes expression of critical genes involved in BC function leading to increased glycaemia, lowered blood insulin levels, defective glucose-stimulated insulin secretion and in the longer term reduced BC mass through apoptosis of a subpopulation of BCs. Nevertheless, glycaemia and blood insulin levels are stabilised after 11 weeks with mutant animals showing long term survival. Bulk RNA-seq and ATAC-seq and single cell RNA-seq on isolated Langerhans islets show that Taf4 loss leads to major remodelling of chromatin accessibility and gene expression not only in targeted BCs, but also alpha and delta cells. One week after Taf4-loss cells with mixed BC, alpha and/or delta cell identities were observed as well as a population trans-differentiating into alpha cells. Trans-differentiation was associated with the concerted action of the Mafb, Mafg, Bptf and Foxp1 transcription factors. Taf4 is therefore essential for BC function and identity and we identify a novel set of factors associated with BC trans-differentiation.
 
Overall design Three ATAC-seq samples from isolated Langerhans islets from wild-type control mice or mice where Taf4 has been inactivated in beta ccells for 1 or 5 weeks.
 
Contributor(s) Kleiber T, Davidson G, Martianov I, Davidson I
Citation(s) 34385420
Submission date May 28, 2020
Last update date Aug 24, 2021
Contact name Irwin Davidson
E-mail(s) irwin67000@gmail.com
Organization name IGBMC
Street address 1 Rue Laurent Fries
City Strasbourg
ZIP/Postal code 67404
Country France
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (3)
GSM4576251 ATAC-seq_Wild type pancreas
GSM4576252 ATAC-seq_TAF-KO pancreas 1 week
GSM4576253 ATAC-seq_TAF-KO pancreas 5 week
This SubSeries is part of SuperSeries:
GSE151366 The TFIID subunit TAF4 is required for pancreatic beta cell function and identity
Relations
BioProject PRJNA635632
SRA SRP265108

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE151363_RAW.tar 8.6 Mb (http)(custom) TAR (of BROADPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.