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| Status |
Public on Oct 02, 2009 |
| Title |
FLX sequencing of Piwi-associated small RNAs extracted from Drosophila ovarian somatic cells (OSC) |
| Organism |
Drosophila melanogaster |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
piRNAs function in silencing retrotransposons by associating with the PIWI proteins, AGO3, Aub, and Piwi, in Drosophila germlines. Bioinformatics analyses of piRNAs in Drosophila ovaries suggested that piRNAs are produced by two systems, the primary processing pathway and the amplification loop, from repetitive genes and piRNA clusters in the genome. The amplification loop occurs in a Dicer-independent, PIWI-Slicer-dependent manner. However, the primary processing pathway remains largely conceptual. Here we show that in ovarian somatic cells, which lack Aub and AGO3 but express Piwi, the primary processing pathway for piRNAs indeed exists.
Keywords: Small RNA profiling by high throughput sequencing
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| Overall design |
Piwi-associated small RNAs were extracted from Drosophila ovarian somatic cells and their deep sequencing was carried out.
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| Contributor(s) |
Saito K, Inagaki S, Mituyama T, Ono Y, Sakota E, Kotani H, Asai K, Siomi H, Siomi MC |
| Citation(s) |
19812547 |
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| Submission date |
Mar 06, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Toutai MITUYAMA |
| Organization name |
National Institute of Advanced Industrial Science and Technology (AIST)
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| Department |
Artificial Intelligence Research Team
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| Lab |
Computational Omics Research Team
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| Street address |
2-4-7 Aomi
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| City |
Koto-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
135-0064 |
| Country |
Japan |
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| Platforms (1) |
| GPL9333 |
454 GS FLX (Drosophila melanogaster) |
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| Samples (1) |
| GSM378200 |
FLX sequencing of Piwi-associated small RNAs extracted from Drosophila ovarian somatic cells (OSC) |
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| Relations |
| SRA |
SRP001366 |
| BioProject |
PRJNA114895 |
| Supplementary data files not provided |
SRA Run Selector |
| Processed data included within Sample table |
| Raw data are available in SRA |
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