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Series GSE15146 Query DataSets for GSE15146
Status Public on Aug 06, 2009
Title mRNA expression in Zfp36L2 knockout E14.5 fetal liver
Organism Mus musculus
Experiment type Expression profiling by array
Summary ZFP36L2, zinc finger protein 36, C3H type-like 2 (also known as Brf2, Erf2, Tis11D) is a member of the tristetraprolin (TTP; Zfp36) family of tandem CCCH zinc finger proteins that can bind to AU-rich elements (AREs) in the 3'-untranslated region of mRNAs, leading to their deadenylation and subsequent degradation. We have generated Zfp36l2 knockout mice. Knockout mice were born at the expected Mendelian frequency, but within several weeks of birth they died rather suddenly with pallor and frequent intestinal hemorrhage. These mice exhibited pancytopenia, decreased hematopoietic progenitor cells from fetal liver and yolk sac, and ineffective hematopoietic stem cells. Since ZFP26L2 is likely to function as an ARE-containing mRNA destabilizing protein, we were interested in identifying any abnormally stabilized transcripts in fetal livers from the Zfp36l2 knockout mice whose protein product may directly or indirectly affect hematopoietic stem cell function.
 
Overall design Total RNA was isolated from individual embryonic day 14.5 fetal livers. Five wild type and five knockout fetal livers were used to generate 5 wild type replicates and 5 knockout replicates. RNA expression from knockout fetal livers was compared to wild type fetal livers.
 
Contributor(s) Stumpo DJ, Broxmeyer HE, Ward T, Cooper S, Hangoc G, Aplan PD, Chung YJ, Yoder MC, Shelley WC, Richfield EK, Ray MK, Blackshear PJ
Citation(s)
  • Stumpo DJ, Broxmeyer HE, Ward T, Cooper S et al. Targeted disruption of Zfp36l2, encoding a CCCH tandem zinc finger RNA-binding protein, results in defective hematopoiesis. Blood 2009 Sep 17;114(12):2401-10. PMID: 19633199
Submission date Mar 06, 2009
Last update date Feb 11, 2019
Contact name NIEHS Microarray Core
E-mail(s) microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (10)
GSM378250 Mouse fetal liver_E14.5_WT_rep1
GSM378251 Mouse fetal liver_E14.5_WT_rep3
GSM378252 Mouse fetal liver_E14.5_WT_rep2
Relations
BioProject PRJNA114911

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15146_RAW.tar 38.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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