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GEO help: Mouse over screen elements for information. |
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Status |
Public on Aug 06, 2009 |
Title |
mRNA expression in Zfp36L2 knockout E14.5 fetal liver |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
ZFP36L2, zinc finger protein 36, C3H type-like 2 (also known as Brf2, Erf2, Tis11D) is a member of the tristetraprolin (TTP; Zfp36) family of tandem CCCH zinc finger proteins that can bind to AU-rich elements (AREs) in the 3'-untranslated region of mRNAs, leading to their deadenylation and subsequent degradation. We have generated Zfp36l2 knockout mice. Knockout mice were born at the expected Mendelian frequency, but within several weeks of birth they died rather suddenly with pallor and frequent intestinal hemorrhage. These mice exhibited pancytopenia, decreased hematopoietic progenitor cells from fetal liver and yolk sac, and ineffective hematopoietic stem cells. Since ZFP26L2 is likely to function as an ARE-containing mRNA destabilizing protein, we were interested in identifying any abnormally stabilized transcripts in fetal livers from the Zfp36l2 knockout mice whose protein product may directly or indirectly affect hematopoietic stem cell function.
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Overall design |
Total RNA was isolated from individual embryonic day 14.5 fetal livers. Five wild type and five knockout fetal livers were used to generate 5 wild type replicates and 5 knockout replicates. RNA expression from knockout fetal livers was compared to wild type fetal livers.
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Contributor(s) |
Stumpo DJ, Broxmeyer HE, Ward T, Cooper S, Hangoc G, Aplan PD, Chung YJ, Yoder MC, Shelley WC, Richfield EK, Ray MK, Blackshear PJ |
Citation(s) |
- Stumpo DJ, Broxmeyer HE, Ward T, Cooper S et al. Targeted disruption of Zfp36l2, encoding a CCCH tandem zinc finger RNA-binding protein, results in defective hematopoiesis. Blood 2009 Sep 17;114(12):2401-10. PMID: 19633199
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Submission date |
Mar 06, 2009 |
Last update date |
Feb 11, 2019 |
Contact name |
NIEHS Microarray Core |
E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
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Organization name |
NIEHS
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Department |
DIR
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Lab |
Microarray Core
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Street address |
111 T.W. Alexander Drive
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City |
RTP |
State/province |
NC |
ZIP/Postal code |
27709 |
Country |
USA |
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Platforms (1) |
GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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Samples (10)
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Relations |
BioProject |
PRJNA114911 |
Supplementary file |
Size |
Download |
File type/resource |
GSE15146_RAW.tar |
38.3 Mb |
(http)(custom) |
TAR (of CEL) |
Processed data included within Sample table |
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