|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Jun 03, 2020 |
Title |
Transcriptomic expression profiles of SPF chicken-lung infected with duck-origin H7N9 subtype avian influenza virus |
Organism |
Gallus gallus |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
In order to elucidate the expression changes of host genes of SPF chickens infected with duck-origin H7N9 subtype avian influenza virus. The lungs of SPF chickens infected with duck-origin H7N9 subtype avian influenza virus were collected and high throughout sequenced. Compared with the control group, there were 740 differentially expressed genes were obtained in infection group, including 602 up-regulated genes and 138 down-regulated genes. The analysis of the GO items showed that the differentially expressed genes were involved in immune responses and inflammatory responses. The results of KEGG analysis showed that 7 pathways were enriched significantly, in those the Toll-like pathway had 11 up-regulated genes, namely IL-6, TLR4, Pik3, IRF7, MD-2, IRF5, MYD88, CD86, STAT1, TLR2, and CCL4. There were 7 up-regulated genes in NOD-like receptor signaling pathway, which were IRF7, CTSB, P2RX7, CYBB, PSTPIP1, HSP90AA1, and NAMPT. In Toll-like signaling pathway,TLR4 was activated by MD-2 after viral infection, and then activated downstream IRF5. At the same time, the NLRP3 inflammasome also played an important role in the process of H7N9 virus infection.
|
|
|
Overall design |
Lung mRNA profiles of SPF chickens infected with duck-origin H7N9 AIV and control were generated by deep sequencing, in triplicate, using Illumina X ten.
|
|
|
Contributor(s) |
Shi S |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
|
Submission date |
Jun 02, 2020 |
Last update date |
Jun 03, 2020 |
Contact name |
Shaohua Shi |
E-mail(s) |
shaohua115@163.com
|
Organization name |
Yangzhou University
|
Department |
College of Veterinary Medicine
|
Street address |
48 East Wenhui Road
|
City |
Yangzhou |
State/province |
Jiangsu |
ZIP/Postal code |
225009 |
Country |
China |
|
|
Platforms (1) |
|
Samples (6)
|
|
Relations |
BioProject |
PRJNA636788 |
SRA |
SRP265640 |
Supplementary file |
Size |
Download |
File type/resource |
GSE151661_1015gene_expression.txt.gz |
1.7 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
|
|
|
|
|