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Status |
Public on Jun 04, 2020 |
Title |
Enteroendocrine cells sense bacterial tryptophan catabolites to activate enteric and vagal neuronal pathways |
Organism |
Danio rerio |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
RNA-seq of isolated zebrafish enteroendocrine cells and other intestinal epithelium cells from germ-free (GF) and conventionalized (CV) zebrafish
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Overall design |
CV and GF TgBAC(cldn15la:EGFP); Tg(neurod1:TagRFP) 8 dpf zebrafish larvae were used for Flow Activated Cell Sorting (FACS) to isolate zebrafish EECs and other IECs. Samples from three independent experimental replicates were performed. 250-580 EECs (n=3 for each CV and GF group) and 100 IECs (n=3 for each CV and GF group) from each experiment were used for library generation and RNA sequencing.
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Contributor(s) |
Ye L, Lickwar CR, Rawls JF, Liddle RA |
Citation(s) |
38577841 |
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Submission date |
Jun 03, 2020 |
Last update date |
Jun 05, 2024 |
Contact name |
Lihua Ye |
E-mail(s) |
lihua.ye@duke.edu
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Phone |
9196600894
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Organization name |
Duke University
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Department |
MGM Department
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Lab |
Rawls lab
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Street address |
213 Research Drive, 327 CARL building
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City |
Durham |
State/province |
NC |
ZIP/Postal code |
27710 |
Country |
USA |
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Platforms (1) |
GPL21741 |
Illumina HiSeq 4000 (Danio rerio) |
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Samples (12)
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Relations |
BioProject |
PRJNA637035 |
SRA |
SRP265808 |