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| Status |
Public on Jun 04, 2020 |
| Title |
Enteroendocrine cells sense bacterial tryptophan catabolites to activate enteric and vagal neuronal pathways |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-seq of isolated zebrafish enteroendocrine cells and other intestinal epithelium cells from germ-free (GF) and conventionalized (CV) zebrafish
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| |
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| Overall design |
CV and GF TgBAC(cldn15la:EGFP); Tg(neurod1:TagRFP) 8 dpf zebrafish larvae were used for Flow Activated Cell Sorting (FACS) to isolate zebrafish EECs and other IECs. Samples from three independent experimental replicates were performed. 250-580 EECs (n=3 for each CV and GF group) and 100 IECs (n=3 for each CV and GF group) from each experiment were used for library generation and RNA sequencing.
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| |
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| Contributor(s) |
Ye L, Lickwar CR, Rawls JF, Liddle RA |
| Citation(s) |
38577841 |
| |
| Submission date |
Jun 03, 2020 |
| Last update date |
Jun 05, 2024 |
| Contact name |
Lihua Ye |
| E-mail(s) |
lihua.ye@duke.edu
|
| Phone |
9196600894
|
| Organization name |
Duke University
|
| Department |
MGM Department
|
| Lab |
Rawls lab
|
| Street address |
213 Research Drive, 327 CARL building
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21741 |
Illumina HiSeq 4000 (Danio rerio) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA637035 |
| SRA |
SRP265808 |
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