|
Status |
Public on Apr 27, 2021 |
Title |
5-hydroxymethylcytosine is Dynamically Regulated During Forebrain Organoid Development and Prematurely Altered in Forebrain Organoids of Alzheimer’s Disease Patients |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing
|
Summary |
5-hydroxymethylcytosine (5hmC) endures dynamic changes during mammalian brain development and its aberrant regulation is known to be associated with numerous neurological diseases such as Alzheimer’s Disease (AD). Recent evidence suggests that key epigenetic changes could occur during neural development long before the onset of neurodegenerative disorders. However, the dynamics of 5hmC during early human brain development and how that contributes to pathogenesis of neurodegeneration, particularly AD pathologies, remain largely unexplored. To investigate this, we utilized the human iPSC-derived organoid model. We derived the 5hmC and transcriptome profiles across healthy forebrain-organoid developmental time points, as well as a patient derived AD organoid time point, allowing us to study brain development at the cellular and molecular levels. In the present study, we identified stage specific differentially hydroxymethylated regions that demonstrated unique acquisition and depletion of 5hmC modifications across development stages. In addition, genes bearing concomitant increases or decreases in both 5hmC and gene expression were enriched in neurobiological processes or early developmental processes respectively. Our AD organoids corroborate both cellular and epigenetic phenotypes previously observed in human AD brains. Importantly, in AD organoids, we identified significant 5hmC alterations at key neurodevelopmental and AD-risk genes, consequently downregulating genes involved in neurodevelopmental and immune response pathways. Collectively our data indicates that, during human fetal neurodevelopment, the precise temporal regulation of 5hmC could modulate key gene expression patterns ensuring that critical neurodevelopmental milestones are achieved. Further, we also demonstrate that premature epigenetic dysregulation of the 5hmC landscape during neuronal development may predispose AD pathogenesis.
|
|
|
Overall design |
Examine the dynamics of 5hmC during early human brain development and how alterations in those dynamics may contribute to Alzheimer's Disease
|
|
|
Contributor(s) |
Kuehner JN, Chen J |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
|
Submission date |
Jun 04, 2020 |
Last update date |
May 01, 2021 |
Contact name |
Janise Nichole Kuehner |
E-mail(s) |
janise.nichole.unger@emory.edu
|
Organization name |
Emory University
|
Department |
Human Genetics
|
Lab |
Bing Yao
|
Street address |
615 Michael St NE
|
City |
Atlanta |
State/province |
GA |
ZIP/Postal code |
30322 |
Country |
USA |
|
|
Platforms (1) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
Samples (48)
|
|
Relations |
BioProject |
PRJNA637330 |
SRA |
SRP265976 |